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首页> 外文期刊>African Journal of Biotechnology >High genetic diversity of Mycospaherella graminicola (Zymoseptoria tritici) from a single wheat field in Tunisia as revealed by SSR markers
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High genetic diversity of Mycospaherella graminicola (Zymoseptoria tritici) from a single wheat field in Tunisia as revealed by SSR markers

机译：SSR标记揭示了突尼斯单一麦田的Mycospaherella graminicola（Zymoseptoria tritici）的高遗传多样性

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Microsatellite?markers were used to assess genetic diversity of?Mycosphaerella graminicola?at a micro geographical scale in Tunisia. Forty five (45) isolates were sampled and assessed using seven pairs of single-locus microsatellite primers not previously tested on populations of?M. graminicola?in Tunisia. Genetic diversity of the field population ranged from 0.403 to 0.555 with an average of 0.484. A high level of genetic diversity was found at a sharp scale throughout the pathogen population tested. Among 45 isolates sampled, 39 different multi locus genotypes (MLG) were identified. Cluster analysis (UPGMA) showed that 86% of the isolates tested were distinct. The high degree of DNA polymorphism, the large number of different molecular genotypes and the pattern of cluster analysis suggest that sexual ascospores and/or asexual spores of a highly mutable local population could have contributed to the genetic diversity of?M. graminicola?in Tunisia.
机译：在突尼斯的微观地理尺度上，使用微卫星标记评估了米氏小球菌的遗传多样性。对四十五（45）个分离株进行采样，并使用七对先前未在？M群体中测试过的单基因座微卫星引物进行评估。 graminicola？在突尼斯。田间种群的遗传多样性范围为0.403至0.555，平均为0.484。在整个测试的病原体种群中发现了高度的遗传多样性。在采样的45个分离株中，鉴定出39个不同的多基因座基因型（MLG）。聚类分析（UPGMA）显示，测试的分离株中有86％是不同的。 DNA多态性高度，大量不同的分子基因型和聚类分析模式表明，高度易变的本地种群的有性子囊孢子和/或无性孢子可能有助于？M的遗传多样性。 graminicola？在突尼斯。

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