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首页> 外文期刊>African Journal of Biotechnology >Genetic divergence of roundup ready (RR) soybean cultivars estimated by phenotypic characteristics and molecular markers
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Genetic divergence of roundup ready (RR) soybean cultivars estimated by phenotypic characteristics and molecular markers

机译：利用表型特征和分子标记估计抗农达大豆品种的遗传差异

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The aim of this study was to estimate the genetic diversity in 74 RR soybean cultivars from different Brazilian breeding programs. Analyzes were based on multivariate statistical techniques from phenotypic characteristics and microsatellite molecular markers (SSR). Ten agronomic traits were used in the analysis of the Euclidean distance, Tocher’s clustering, UPGMA clustering and principal component analysis. Eighty-six of 100 SSR primer-pairs studied were selected based on their polymorphism information content, and analyzed using Jaccard Coefficient and UPGMA clustering method. The cultivars were clustered into seven groups according to the UPGMA and Tocher’s methods, based on agronomic traits, while molecular analysis identified six groups. The phenotypic distances ranged from 0.46 to 9.79 and the dissimilarity measurements, based on SSR molecular markers, ranged from 0.07 to 0.73. Both results from agronomic traits and molecular markers showed that there is genetic variability among the RR cultivars and that the Monsanto breeding program has the most divergent germplasm. The analyzed agronomic traits and the chosen SSR markers were effective in assessing the genetic diversity among genotypes, besides proving to be useful for characterizing genetic variability of soybean germplasm.
机译：这项研究的目的是评估来自不同巴西育种计划的74个RR大豆品种的遗传多样性。分析基于表型特征和微卫星分子标记（SSR）的多元统计技术。十个农艺性状用于欧氏距离分析，托赫聚类，UPGMA聚类和主成分分析。根据多态性信息含量，从研究的100个SSR引物对中选择86个，并使用Jaccard系数和UPGMA聚类方法进行分析。根据农艺性状，根据UPGMA和Tocher的方法，将品种分为7个组，而分子分析确定了6个组。表型距离范围为0.46至9.79，基于SSR分子标记的相异性测量范围为0.07至0.73。农艺性状和分子标记的结果均表明，RR品种之间存在遗传变异，孟山都育种计划的种质最多。所分析的农艺性状和选择的SSR标记物对于评估基因型之间的遗传多样性是有效的，除了证明可用于表征大豆种质的遗传变异性。

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