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首页> 外文期刊>African Journal of Biotechnology >Application of random amplified polymorphic DNA (RAPD) markers to identify Taxus chinensis var. mairei cultivars associated with parthenogenesis
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Application of random amplified polymorphic DNA (RAPD) markers to identify Taxus chinensis var. mairei cultivars associated with parthenogenesis

机译：应用随机扩增多态性DNA（RAPD）标记鉴定红豆杉变种。与孤雌生殖有关的梅里品种

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The random amplified polymorphic DNA (RAPD) technique has been widely applied to identify different varieties of plants for molecular breeding. However, application of RAPD markers to identify parthenogenesis in plants has not been reported. In this investigation, we used pedigree and RAPD markers to differentiate different Taxus chinensis var. mairei cultivars that were associated with parthenogenesis. Among 180 and eighty RAPD primers used, 108 primers generated RAPD bands from genomic DNA of T. chinensis var. mairei cultivars (“Jingzhou” and “Baokang”). Six hundred and thirty-three RAPD loci bands were produced and used to identify eight cultivars with unique banding patterns. Pedigree and RAPD data demonstrated that T. chinensis var. mairei cultivars with parthenogenesis were separated from others in both “Jingzhou” and “Baokang” cultivars. These results provide evidence for identification of parthenogenesis and confirm that the RAPD technique is especially suitable for identification of T. chinensis var. mairei cultivars for agricultural purposes.
机译：随机扩增多态性DNA（RAPD）技术已广泛应用于鉴定用于分子育种的不同植物品种。但是，尚未报道使用RAPD标记物鉴定植物中的孤雌生殖。在这项调查中，我们使用谱系和RAPD标记来区分不同的南方红豆杉变种。与孤雌生殖相关的玛莱品种。在180种RAPD引物和80种RAPD引物中，108种引物从中华T的基因组DNA产生RAPD条带。 mairei品种（“荆州”和“保康”）。产生了633个RAPD基因座带，并用于鉴定八个具有独特带型的品种。谱系和RAPD数据表明，T。chinensis var。在“荆州”和“保康”两个品种中，具有孤雌生殖的玛莱品种被分离。这些结果为单性生殖的鉴定提供了证据，并证实了RAPD技术特别适用于中华绒螯蟹的鉴定。农业用途的mairei品种。

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