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首页> 外文期刊>African Journal of Biotechnology >Molecular characterization of traditional and improved rice cultivars based on random amplified polymorphic DNAs (RAPDs) markers
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Molecular characterization of traditional and improved rice cultivars based on random amplified polymorphic DNAs (RAPDs) markers

机译：基于随机扩增多态性DNA（RAPD）标记的传统和改良水稻品种的分子表征

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Information of genetic similarities and diversity among superior rice cultivars is needed for potential rice breeding programs and origin of plant lines. Genetic polymorphism and relationships among 35 commercial varieties and primitive cultivars of Rice (Oryza sativa?L.) were established by using random amplified polymorphic DNAs (RAPDs) primers. Polymerase chain reaction (PCR) with 14 primers produced a total of 126 different marker bands, of which 82.5% were polymorphic. The size range of the amplified DNAs was mostly between 0.2 and 3.63 kbp. Cluster analysis using an un-weighted pair-group method with arithmetic averages (UPGMA) was used to group the cultivars. Cluster analysis placed most of the aromatic cultivars close to each other showing a high level of genetic relatedness. Lower levels of polymorphism in aromatic cultivars of rice indicated that there is a basic similarity among basmati cultivars used in this study, which is to be expected due to their same ancestors and selection for similar characteristics. The RAPD analysis offered a simple and quick method for the assessment of variability between different cultivars.
机译：潜在的水稻育种计划和植物系起源需要优质水稻品种之间遗传相似性和多样性的信息。利用随机扩增的多态性DNAs（RAPDs）引物，建立了35个水稻商品化品种和原始品种之间的遗传多态性和相关性。用14个引物进行的聚合酶链反应（PCR）产生了126条不同的标记带，其中82.5％是多态性的。扩增的DNA的大小范围主要在0.2至3.63kbp之间。使用具有算术平均值的非加权成对组方法（UPGMA）进行聚类分析，对品种进行分组。聚类分析表明，大多数芳香品种彼此靠近，显示出高度的遗传相关性。水稻芳香品种中较低的多态性水平表明该研究中使用的印度香米品种之间存在基本相似性，这是可以预期的，因为它们的祖先相同，并且选择了相似的特性。 RAPD分析提供了一种简单快速的方法来评估不同品种之间的变异性。

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