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Genome polymorphism markers and stress genes expression for identifying turf species

机译:基因组多态性标记和胁迫基因表达鉴定草皮种类

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Reactive oxygen species (ROS) are produced in both stressed and unstressed cells. Superoxide dismutase (SOD) and phenylalanine ammonia lyase (PAL) play an important role in the defense against ROS. Eight different turf grass species were used in order to detect their ability to withstand environmental stress through investigating SOD and PAL gene expression and also the genetic relationship among them using random amplified polymorphic DNA (RAPD) molecular markers. The levels of expression of PAL, SOD genes and mRNA varied with the type of turf; both PAL and SOD gene expressions were low in cold season turf grasses (kentucky blue grass and fine fescue), moderate for bermuda hybrids (tifgreen and tifway) and high in Paspalum vaginatum. Primer 3 (UBC-245) can be used to distinguish between Paspalum species, also between common burmuda (Cynodon dactylon) and burmuda hybrids. It was concluded that hot season genera can withstand environmental stress more than cold season ones since they have more SOD and PAL gene expressions. Also, DNA markers can be used to differentiate between different turf genera which are hard to be differentiated morphologically.
机译:在压力细胞和非压力细胞中都会产生活性氧(ROS)。超氧化物歧化酶(SOD)和苯丙氨酸氨裂合酶(PAL)在防御ROS中起着重要作用。使用八种不同的草皮草种,以通过调查SOD和PAL基因的表达来检测其抵御环境胁迫的能力,并使用随机扩增多态性DNA(RAPD)分子标记来检测它们之间的亲缘关系。 PAL,SOD基因和mRNA的表达水平随草皮类型的不同而变化。在寒冷季节的草皮草(肯塔基蓝草和优质羊茅)中,PAL和SOD基因表达均较低,在百慕大杂种(tifgreen和tifway)中,PAL和SOD基因表达较高,而在阴道雀Pa中则较高。引物3(UBC-245)可用于区分Paspalum物种,也可区分常见的burmuda(Cynodon dactylon)和burmuda杂种。结论是,热季属比SOD和PAL基因表达更多,因此比冷季能承受更多的环境压力。同样,DNA标记可用于区分很难在形态上区分的不同草皮属。

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