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首页> 外文期刊>African Journal of Biotechnology >Random amplified polymorphic DNA (RAPD) based assessment of genetic relationships among some Zimbabwean sorghum landraces with different seed proanthocyanidin levels
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Random amplified polymorphic DNA (RAPD) based assessment of genetic relationships among some Zimbabwean sorghum landraces with different seed proanthocyanidin levels

机译：基于随机扩增多态DNA（RAPD）的一些种子原花色素水平不同的津巴布韦高粱地方种之间的遗传关系评估

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Knowledge of genetic distances between genotypes is important for efficient organization and conservation of plant genetic resources for crop improvement programs. In this study genetic distances between genotype pairs (complements of Jaccard's similarity coefficient) were estimated from Random Amplified Polymorphic DNA (RAPD) data collected from 48 Zimbabwean sorghum landraces. These varieties showed variation in their seed proanthocyanidin (PAs) levels with 16 and 29 of them having detectable and non-detectable PA levels respectively. RAPDs revealed considerable genetic variation between the varieties used and 2.7 polymorphisms per primer were obtained.? Ninety nine polymorphic RAPD bands were used to calculate genetic distances and the mean genetic distance between the genotypes was 0.494 (± 0.113) with a range of 0.051 to 0.761. A multidimensional scaling (MDS) plot of the distance matrix revealed two distinct clusters of cultivated and wild sorghums. No clustering of genotypes according to their seed proanthocyanidin levels was revealed by MDS analysis; also the mean genetic distances of genotypes in the low, medium and high PA categories were not different from each other and none of them was significantly different from the mean genetic distances between all the groups. The RAPD markers used in the present study could not distinguish between sorghums with different PA levels in their seeds; however, the protocol established could be useful in further analysis of this trait in near isogenic lines.
机译：了解基因型之间的遗传距离对于有效组织和保护植物遗传资源以进行作物改良计划很重要。在这项研究中，基因型对之间的遗传距离（Jaccard相似系数的补语）是根据从48个津巴布韦高粱地方品种中收集的随机扩增多态性DNA（RAPD）数据估算的。这些变种的种子原花青素（PA）水平显示出变​​化，其中16个和29个分别具有可检测和不可检测的PA水平。 RAPD揭示了所用品种之间的显着遗传变异，每个引物获得2.7个多态性。使用九十九个多态性RAPD条带来计算遗传距离，基因型之间的平均遗传距离为0.494（±0.113），范围为0.051至0.761。距离矩阵的多维标度（MDS）图显示了两个不同的栽培高粱和野生高粱群。通过MDS分析未发现根据其种子原花色素水平的基因型聚类。低，中和高PA类别的基因型的平均遗传距离也没有差异，并且与所有组之间的平均遗传距离均无显着差异。本研究中使用的RAPD标记无法区分种子中PA含量不同的高粱。然而，建立的协议可能有助于进一步分析近等基因系中的该性状。

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