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首页> 外文期刊>African Journal of Biotechnology >Comparison of protease production from newly isolated bacterial strains T5, T3 and H3 after immobilization
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Comparison of protease production from newly isolated bacterial strains T5, T3 and H3 after immobilization

机译：固定后从新分离的细菌菌株T5，T3和H3生产蛋白酶的比较

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Protease has gained a very important position in many industries such as food, pharmaceutical, chemical and leather industries. In this research, protease was obtained from bacteria. The bacterial strain was obtained from soil which was collected from different areas of Lahore, Pakistan. Fermentation medium (by using sub-merged fermentation technique) was incubated for 48 h at 37°C temperature and agitation speed of 200 rpm. The protease was partially purified with 70% ammonium sulphate. Four different supports were used for the immobilization of the bacterial protease by physical adsorption method. When partially purified protease was immobilized on Amberlite (XAD 761), its production amount was 371.42%? (52 U/ml/g support) with strain T5; 208.33% (25 U/ml/g support) with strain T3 and 342.24% (64 U/ml/g support) with strain H3; when it was immobilized on Duolite (A568), its amount of production was 314.28% (44 U/ml/g support) with strain T5; 225% (27 U/ml/g support) with strain T3 and 395.72% (74 U/ml/g support) with strain H3; when it was immobilized on Lewatit (VPOC 1600), the amount of production was 450% (63 U/ml/g support) with strain T5; 541.66% (65 U/ml/g support) with strain T3 and 320.85% (60 U/ml/g support) with strain H3. But, when it was immobilized on Pentynyl Dextran (NT4L360), its amount of production was 271.42% (38 U/ml/g support) with strain T5, 3483.33% (418 U/ml/g support) with strain T3 and 304.81% (57 U/ml/g support) with strain H3. Immobilized protease from bacterial strain T3 had the highest immobilizing activity of 3483.33% (418 U/ml/g support). This immobilized protease with the highest activity could be used in food, pharmaceutical and leather industries.
机译：蛋白酶在许多行业（例如食品，制药，化学和皮革行业）中都占有非常重要的地位。在这项研究中，蛋白酶是从细菌中获得的。细菌菌株是从土壤中获得的，土壤是从巴基斯坦拉合尔不同地区收集的。发酵培养基（通过使用深层发酵技术）在37°C和200 rpm的搅拌速度下孵育48小时。蛋白酶用70％硫酸铵部分纯化。使用四种不同的载体通过物理吸附法固定细菌蛋白酶。将部分纯化的蛋白酶固定在Amberlite（XAD 761）上时，其产量为371.42％。 （52 U / ml / g载体），菌株T5;菌株T3为208.33％（支持25 U / ml / g），菌株H3为342.24％（支持64 U / ml / g）;当将其固定在Duolite（A568）上时，菌株T5的产量为314.28％（44 U / ml / g载体）。 T3菌株为225％（27 U / ml / g载体），H3菌株为395.72％（74 U / ml / g载体）;当将其固定在Lewatit（VPOC 1600）上时，菌株T5的产量为450％（63 U / ml / g载体）。 T3菌株为541.66％（支持65 U / ml / g），H3菌株为320.85％（支持60 U / ml / g）。但是，当将其固定在戊炔葡聚糖（NT4L360）上时，菌株T5的产量为271.42％（38 U / ml / g载体），菌株T3为3483.33％（418 U / ml / g载体）和304.81％ （57 U / ml / g载体）和H3菌株。来自细菌菌株T3的固定的蛋白酶具有3483.33％的最高固定活性（418U / ml / g支持物）。这种具有最高活性的固定化蛋白酶可用于食品，制药和皮革工业。

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