• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>African Journal of Biotechnology >Cost effective medium for in vitro propagation of Tanzanian cassava landraces
【24h】

Cost effective medium for in vitro propagation of Tanzanian cassava landraces

机译：坦桑尼亚木薯地方品种体外繁殖的经济有效培养基

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Cassava (Manihot esculenta Crantz) is a staple food for over 800 million people in the tropics. However, its production is constrained by an inadequate supply of clean planting materials. Tissue culture carried out in laboratories is one established method for the mass production of clean planting materials. However, the cost of conventional tissue culture is high and the cassava industry would benefit from an alternative means of propagation. In the current study, a cost-effective protocol for micropropagation of the farmer-preferred cassava landraces ‘Kibandameno’ and ‘Paja la mzee’ in Tanzania was evaluated. Ammonium fertilizer, potassium fertilizer, epsom salt, monopotassium phosphate and calcinit were used as alternative source to conventional Murashige and Skoog (MS) macronutrients, while Stanes Iodized Microfood? was used as alternative to MS micronutrients. Nodal cuttings of the 2 cultivars were initiated in either conventional MS or cost-effective medium supplemented with 20 g/l table sugar and 3 g/L agar. Conventional MS was used as the control in this study. Four parameters namely plant height, number of leaves, number of nodes and number of roots were recorded from the two media and the differences were determined. For all 4 parameters, both cultivars performed better in the cost-effective medium as compared to conventional MS. More than 75% of plantlets acclimatized to greenhouse conditions from both types of media survived. The cost of production of cassava plantlets in both types of media was then calculated and compared. The use of the cost effective medium led to a cost reduction of 93% over conventional MS medium, which makes it a feasible and attractive alternative for growers.
机译：木薯（Manihot esculenta Crantz）是热带地区超过8亿人的主食。但是，其生产受到清洁种植材料供应不足的限制。在实验室中进行的组织培养是大规模生产清洁种植材料的一种成熟方法。但是，常规组织培养的成本很高，木薯产业将从另一种繁殖方式中受益。在当前的研究中，评估了坦桑尼亚偏爱农民首选的木薯地方品种“ Kibandameno”和“ Paja la mzee”的经济有效方案。铵肥，钾肥，泻盐，磷酸二氢钾和方解石被用作常规Murashige和Skoog（MS）大型营养素的替代来源，而Stanes碘化微食品？被用作MS微量营养素的替代品。在常规MS或经济高效的培养基中添加2 g / l食糖和3 g / L琼脂后，开始对2个品种的节间插条。常规MS被用作本研究的对照。从这两种介质中记录了四个参数，即植物高度，叶数，节数和根数，并确定了差异。对于所有4个参数，与常规MS相比，这两个品种在具有成本效益的培养基中均表现更好。从这两种类型的培养基适应温室条件的苗中，超过75％存活。然后计算并比较了两种培养基中木薯苗的生产成本。与传统的MS培养基相比，使用具有成本效益的培养基可将成本降低93％，这使其成为种植者可行且有吸引力的替代方法。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号