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首页> 外文期刊>African Journal of Biotechnology >Screening for total antioxidant activity, flavonoids and phenolics variability in forty-five accessions of roselle (Hibiscus sabdariffa L.)
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Screening for total antioxidant activity, flavonoids and phenolics variability in forty-five accessions of roselle (Hibiscus sabdariffa L.)

机译：筛选45种玫瑰茄（Hibiscus sabdariffa L.）中的总抗氧化剂活性，类黄酮和酚类变异性

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Build-up of free radicals in the human body can cause oxidative stress which may invariably lead to degenerative diseases and eventual death. Antioxidants such as polyphenols and flavonoids found in fruits and vegetables have been shown to protect the body against the damaging effects of these free radicals. Roselle contains high amounts of antioxidants and its leaves are often used for sauces or in herbal preparations to treat certain ailments. Several landraces of roselle occur in Ghana but have not been screened for antioxidant activity. Leaf samples of roselle acessions were harvested 60 days after sowing, freeze dried and analysed for total phenolic content (TPC), total flavonoid content (TFC) as well as total antioxidant activity (TAA) using UV-VIS Spectrophotometer (Shimadzu, 1201, Japan). No statistically significant differences were observed in TPC which ranged from 20 ± 0.6 μg/g (RNL) to 90 ± 0.6 μg/g (Sob-4). TFC and TAA in the leaf samples; however, showed statistically significant variation and ranged from 10.04 ± 0.31 μg/g (WH-S2V) to 49.79 ± 0.48 μg/g (Don-1) and 37.48% (Don-4) to 58.58% (WNL-HS), respectively. Higher leaf phenolic or flavonoid content did not necessarily translate into a higher antioxidant activity. This suggests that other forms of antioxidants other than phenolics or flavonoids might be responsible free radical scavenging activity in roselle leaves. Nevertheless, very high free radical percentage inhibition observed in the roselle leaves makes it an excellent material for mitigation against the adverse effect of free radicals. Nine promising accessions with high free radical scavenging activity have been identified for further improvement.
机译：人体中自由基的积累会引起氧化应激，这必然会导致退行性疾病并最终导致死亡。水果和蔬菜中发现的抗氧化剂，例如多酚和类黄酮，已被证明可以保护人体免受这些自由基的破坏作用。玫瑰茄含有大量的抗氧化剂，其叶子通常用于调味料或草药制剂中，以治疗某些疾病。在加纳出现了几种玫瑰茄地方品种，但尚未筛选出抗氧化剂活性。播种后60天收获玫瑰茄小叶的叶片样品，冷冻干燥并使用UV-VIS分光光度计（Shimadzu，1201，日本）分析总酚含量（TPC），总黄酮含量（TFC）和总抗氧化剂活性（TAA）。 ）。在TPC中未观察到统计学上的显着差异，范围从20±0.6μg/ g（RNL）到90±0.6μg/ g（Sob-4）。叶子样品中的TFC和TAA;然而，显示出统计学上的显着变化，范围从10.04±0.31μg/ g（WH-S2V）到49.79±0.48μg/ g（Don-1）和37.48％（Don-4）至58.58％（WNL-HS） 。较高的叶片酚或类黄酮含量不一定转化为较高的抗氧化活性。这表明除酚醛或类黄酮以外的其他形式的抗氧化剂可能是玫瑰茄叶片中自由基清除的原因。然而，在玫瑰茄叶片中观察到的很高的自由基百分抑制作用使其成为减轻自由基不利影响的极佳材料。已经鉴定出九种具有高自由基清除活性的有希望的登录品以进一步改进。

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