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首页> 外文期刊>African Journal of Biotechnology >Validated high performance liquid chromatographic (HPLC) method for analysis of zerumbone in plasma
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Validated high performance liquid chromatographic (HPLC) method for analysis of zerumbone in plasma

机译：经验证的高效液相色谱（HPLC）方法分析血浆中的蛇骨酮

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摘要

Zerumbone (ZER) is a sesquiterpene derived from Zingiber zerumbet smith, family Zingiberaceae. It has been shown to possess anti-cancer and apoptosis-inducing properties against various human tumour cells as well as?in vivo?against a number of induced malignancies in mice. In this study a?simple, specific and accurate high performance liquid chromatographic method for determination of ZER in micro-volumes human plasma (|?1.5 ml) was developed and validated. ZER and its analogue α-Humulene as internal standard were easy recovered by simple one step plasma protein precipitation using acetonitrile and separated in isocratic mobile phase, on reverse phase-C18?column. The effluent was monitored by Photodiode Array (PDA) detector and at a flow rate of 1.0 ml/min. The linearity of proposed method was 2 – 15 μg/ml. The intra-day and inter-day coefficient of variation and percent error values of the method were less than 15% and mean recovery was more than 90% for both ZER and α-Humulene. This method was found to be precise, specific, accurate and robust for detection and analysis of ZER in human plasma.
机译：Zerumbone（ZER）是一种倍半萜烯，得自姜科姜科姜（Zingiber zerumbet smith）。它已显示出对各种人类肿瘤细胞具有抗癌和诱导凋亡的特性，并且在体内具有抗小鼠多种恶性肿瘤的作用。在这项研究中，开发并验证了一种简单，特异和准确的高效液相色谱法测定微量人血浆（| 1.5 ml）中的ZER。 ZER及其类似物α-Humulene作为内标很容易通过乙腈进行的一步一步血浆蛋白沉淀而回收，并在等度流动相中于C18反相色谱柱上分离。通过光电二极管阵列（PDA）检测器以1.0 ml / min的流速监测流出物。所提出方法的线性为2 – 15μg/ ml。该方法的日内和日间变异系数以及百分比误差值均小于15％，ZER和α-Humulene的平均回收率均大于90％。发现该方法对于检测和分析人血浆中的ZER是精确，特异性，准确和可靠的。

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