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首页> 外文期刊>African Journal of Biotechnology >Determination of genetic diversity among Turkish durum wheat landraces by microsatellites
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Determination of genetic diversity among Turkish durum wheat landraces by microsatellites

机译：用微卫星测定土耳其硬粒小麦地方品种的遗传多样性

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摘要

Wheat landraces represent an important source of genetic variation that can be used for future wheat breeding program. The rich wheat landraces from Turkey have not been sufficiently analyzed genetically. For this reason,?genetic diversity and relationship of the landraces must be determined. In this study, genetic diversity of 20 durum wheat landraces collected from different regions of Turkey were determined using 12 highly polymorphic microsatellite (SSR) markers. Genomic DNAs were isolated from ten accessions of all durum wheat landraces and specific regions were amplified with the polymerase chain reaction by using microsatellite markers. Polymorphic bands changed 4 to 9 per each SSR locus and the most polymorphic SSR loci were Wms 18, Wms 155, Xgwm 166 and Stm 578. The dendrogram showed that durum wheat landraces can be divided into two major groups. The coefficient of similarity among all germplasm ranged from 0.35 to 0.74. According to the estimated matrices and relationship among landraces accessions, it was determined that the 46847-47341, 31738-52841 and 31634-47923 were genetically the closest. The farthest genotypes were 52835-1680. The results showed that durum wheat landraces have high genetic variability and microsatellite DNA markers could be successfully employed for revealing the variability.
机译：小麦地方品种是遗传变异的重要来源，可用于未来的小麦育种计划。尚未对土耳其丰富的小麦地方品种进行遗传分析。因此，必须确定地方品种的遗传多样性和亲缘关系。在这项研究中，使用12个高度多态性微卫星（SSR）标记确定了从土耳其不同地区收集的20种硬质小麦地方品种的遗传多样性。从所有硬粒小麦地方品种的十份中分离出基因组DNA，并使用微卫星标记通过聚合酶链反应扩增特定区域。每个SSR基因座的多态性谱带变化为4到9，最多态的SSR基因座是Wms 18，Wms 155，Xgwm 166和Stm578。树状图显示硬粒小麦长白种可分为两个主要组。所有种质之间的相似系数在0.35至0.74之间。根据估计的矩阵和地方种质之间的关系，确定46847-47341、31738-52841和31634-47923在遗传上最接近。最远的基因型是52835-1680。结果表明，硬粒小麦本地种具有较高的遗传变异性，微卫星DNA标记可以成功地揭示变异性。

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