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首页> 外文期刊>African Journal of Biotechnology >Enhancement of callus induction and regeneration efficiency from embryo cultures of Datura stramonium by adjusting carbon sources and concentrations
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Enhancement of callus induction and regeneration efficiency from embryo cultures of Datura stramonium by adjusting carbon sources and concentrations

机译：通过调节碳源和碳浓度提高曼陀罗胚胎培养物中愈伤组织的诱导和再生效率

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Carbon source in the medium is considered to be an essential component for the high production costs of callus and plantlets in tissue culture. We report here the establishment of an efficient tissue culture cycle (callus induction and plant regeneration) for?Datura stramonium?by adjusting carbon sources and concentrations. Embryo explants of?D. stramonium?L.?were cultured?in vitro?with six different carbon sources (sucrose, glucose, fructose, galactose, maltose and lactose) and four concentrations (1, 2, 3 and 4%) for callus induction. After that, the best carbon sources for regeneration were investigated with four different carbon sources (sucrose, glucose, fructose and sorbitol) and four concentrations (1, 2, 3 and 4%). The best sugar for callus induction was 2% lactose. The medium containing 3% glucose shows the higher regeneration.
机译：培养基中的碳源被认为是组织培养中愈伤组织和小植株高生产成本的重要组成部分。我们在这里报告通过调节碳源和碳浓度为“曼陀罗”建立了有效的组织培养周期（愈伤组织诱导和植物再生）。 D.的胚胎外植体用6种不同的碳源（蔗糖，葡萄糖，果糖，半乳糖，麦芽糖和乳糖）和4种浓度（1、2、3和4％）体外诱导培养了Stramonium？L.。之后，用四种不同的碳源（蔗糖，葡萄糖，果糖和山梨糖醇）和四种浓度（1、2、3和4％）研究了再生的最佳碳源。诱导愈伤组织的最佳糖是2％乳糖。含3％葡萄糖的培养基显示出更高的再生。

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