首页> 外文期刊>African Journal of Biotechnology >High-yield cellulase production in solid-state fermentation by Trichoderma reesei SEMCC-3.217 using water hyacinth (Eichhornia crassipes)
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High-yield cellulase production in solid-state fermentation by Trichoderma reesei SEMCC-3.217 using water hyacinth (Eichhornia crassipes)

机译:使用水葫芦(Eichhornia crassipes)通过里氏木霉SEMCC-3.217在固态发酵中高产纤维素酶的生产

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In this study, the strain?Trichoderma reesei?SEMCC-3.217 was used for producing cellulase in solid-state fermentation with?water hyacinth (Eichhornia crassipes). The results of fractional factorial design showed that, the addition amount of wheat bran, (NH4)2SO4, CaCl2?and Tween 80 had significant effect on the cellulase production. Then, these four factors were selected for further optimization by central composite design for the yield of cellulase. The statistical analysis of the results showed that, the optimum composition were: 5 g of substrate containing 3.9 g water hyacinth, 1% corn steep liquor, 1% soybean meal, 0.2% NH4NO3, 0.2% KH2PO4, 0.08% MgSO4·7H2O, 2.8% (NH4)2SO4, 1.5% urea, 13.9% wheat bran, 0.08% ZnSO4·7H2O, 0.08% FeCl2?0.05% CaCl2, 0.08% NaNO3,?0.08% KCl and 0.27% (v/v) Tween-80. Under these conditions, the cellulase production was 4-fold increased (13.4 FPIU/g dry solid) compared with the initial level (3.4 FPIU/g dry solid) after 7 days of fermentation in a 250 ml Erlenmeyer flask.
机译:在这项研究中,使用里氏木霉菌株SEMCC-3.217在水葫芦(凤眼棘)的固态发酵中生产纤维素酶。分数阶乘设计结果表明,麦麸,(NH4)2SO4,CaCl2α和吐温80的添加量对纤维素酶的生产有显着影响。然后,通过中央复合设计来选择这四个因素,以进一步优化纤维素酶的产量。结果的统计分析表明,最佳组合物为:5 g含3.9 g水葫芦的底物,1%玉米浆,1%豆粕,0.2%NH4NO3、0.2%KH2PO4、0.08%MgSO4·7H2O,2.8 %(NH4)2SO4、1.5%尿素,13.9%麦麸,0.08%ZnSO4·7H2O,0.08%FeCl2→0.05%CaCl2、0.08%NaNO3,〜0.08%KCl和0.27%(v / v)Tween-80。在这些条件下,在250 ml锥形瓶中发酵7天后,纤维素酶的产量比初始水平(3.4 FPIU / g干燥固体)增加了4倍(13.4 FPIU / g干燥固体)。

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