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首页> 外文期刊>African Journal of Biotechnology >DNA landmarks for genetic diversity assessment in tea genotypes using RAPD markers
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DNA landmarks for genetic diversity assessment in tea genotypes using RAPD markers

机译：利用RAPD标记对茶基因型进行遗传多样性评估的DNA标志

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Tea (Camellia sinensis)?is one of the most important non-alcoholic beverages of the world. Natural genetic diversity in tea has been reduced due to continue selection in favor of desirable traits.?The?present study was conducted to estimate genetic diversity in tea genotypes cultivated in Pakistan using 20 randomly amplified polymorphic DNA (RAPD) primers.?75?tea accessions from 13 tea genotypes were analyzed. Genetic distance estimates ranged from 0-100% showing high level of diversity among?screened genotypes. Unweighted pair group method using arithmetic averages (UPGMA) based cluster analyses of RAPD data clustered all the genotypes into five main groups which showed mixed grouping profile of the samples. However, samples from different plants of particular genotypes were segregated into independent sub-clusters. Broad and narrow leaved genotypes were accommodated in separate sub-clusters. Similarly,?samples from narrow leaved genotypes collected from different sites were grouped in different main clusters reflecting the geographical origins of tea samples. Results obtained confirm that RAPD methodology is practically applicable for evaluation of genetic diversity and relationship in tea genotypes.
机译：茶（山茶）是世界上最重要的非酒精饮料之一。茶的自然遗传多样性由于继续选择有利于期望的性状而减少了。本研究旨在评估巴基斯坦使用20种随机扩增多态性DNA（RAPD）引物培育的茶基因型的遗传多样性。？75？tea分析了13种茶基因型的种质。遗传距离的估计范围为0-100％，这表明筛选出的基因型之间具有高度的多样性。使用基于算术平均值（UPGMA）的RAPD数据聚类分析的非加权成对组方法将所有基因型分为五个主要组，这五个主要组显示了样本的混合分组情况。但是，将来自特定基因型不同植物的样品分离到独立的亚类中。阔叶和窄叶基因型被容纳在单独的亚群中。同样，从不同地点采集的窄叶基因型样品被分为不同的主要簇，反映了茶样品的地理起源。获得的结果证实，RAPD方法学可实际用于评估茶基因型的遗传多样性和相关性。

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