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首页> 外文期刊>African Journal of Biotechnology >Isolation and characterization of polymorphic microsatellite markers from flax (Linum usitatissimum L.)
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Isolation and characterization of polymorphic microsatellite markers from flax (Linum usitatissimum L.)

机译：亚麻（Linum usitatissimum L.）多态性微卫星标记的分离与鉴定

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摘要

Flax (Linum usitatissimum?L.) is the third largest natural fiber crop and one of the five major oil crops in the world. Flax can be used as model plant for the bast fiber plants because of its small size and self pollination characters. Development of highly polymorphic co-dominant markers will be important for the molecular and genomic research in flax. Two microsatellite-enriched flax genomic libraries were constructed for trinucleotide TTC and ATC motifs. A total of 206 new microsatellite-containing sequences were identified and classified. Thirty eight polymorphic microsatellite markers were characterized and evaluated in eight cultivars from different countries and regions. These loci produced 2 to 12 alleles per locus with an average of 3.395. The observed and expected heterozygosities ranged from 0.000 to 1.000 (mean 0.257) and from 0.125 to 0.950 (mean 0.516), respectively. The mean?polymorphic information content (PIC) value over 38 loci was 0.429, with 13 loci having PIC greater than 0.5. These novel polymorphic microsatellite loci will be useful in genetic linkage map construction, germplasm classification and identification, gene identification and QTL mapping, and marker-assisted selection in breeding of?L. usitatissimum.
机译：亚麻（Linum usitatissimum？L。）是世界第三大天然纤维作物，也是世界五种主要油料作物之一。亚麻由于其体积小和自花授粉的特性，可以用作韧皮纤维植物的模型植物。高度多态共显性标记的开发对于亚麻的分子和基因组研究将是重要的。构建了两个富含微卫星的亚麻基因组文库，用于三核苷酸TTC和ATC图案。总共确定并分类了206个新的含微卫星序列。在来自不同国家和地区的八个品种中鉴定并评估了38个多态微卫星标记。这些基因座每个位点产生2至12个等位基因，平均为3.395。观察到和预期的杂合度分别为0.000至1.000（平均0.257）和0.125至0.950（平均0.516）。 38个位点的平均多态信息含量（PIC）值为0.429，其中13个位点的PIC大于0.5。这些新颖的多态微卫星基因座将用于遗传连锁图谱的构建，种质分类和鉴定，基因鉴定和QTL作图以及标记辅助育种的选择。普通话

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