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首页> 外文期刊>African Journal of Biotechnology >In vitro propagation of Ceropegia thwaitesii Hook- an endemic species of Western Ghats of Tamil Nadu, India
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In vitro propagation of Ceropegia thwaitesii Hook- an endemic species of Western Ghats of Tamil Nadu, India

机译：印度泰米尔纳德邦西高止山脉的特有种印度猪尾柏（Ceropegia thwaitesii Hook）的体外繁殖

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摘要

In vitro?propagation of?Ceropegia thwaitesii?Hook?(Asclepiadaceae), an endemic plant species of Western Ghats, Tamil Nadu (India), was carried out. Auxiliary bud explants were cultured on Murashige and Skoog (MS) medium fortified with cytokinins (KIN), 6-benzyl adenine (BA), 2-isopentenyladenine (2-iP) and thidiazuron (TDZ) in various concentrations and in combination with auxins indole-3-butyric acid (IBA), indole-3-acetic acid (IAA) and naphthalene acetic acid (NAA). High frequency of shoot bud proliferation and multiplication was observed on KIN (13.94 μm) + IAA (28.54 μm). Rhizogenesis was observed on MS medium supplemented with IBA?2.46 μm?and plantlets produced through micropropagation were hardened with the survival success of 73.33%. The efficient Micropropagation regeneration protocol developed would aid?ex situ?conservation of this endemic species.
机译：进行了泰米尔纳德邦（印度）西高止山脉的特有植物物种苏氏紫薇（Ceropegia thwaitesii？Hook）（Asclepiadaceae）的体外繁殖。辅助芽外植体在强化有细胞分裂素（KIN），6-苄基腺嘌呤（BA），2-异戊烯腺嘌呤（2-iP）和噻二唑酮（TDZ）的Murashige和Skoog（MS）培养基上培养，并与生长素吲哚组合使用-3-丁酸（IBA），吲哚-3-乙酸（IAA）和萘乙酸（NAA）。在KIN（13.94μm）+ IAA（28.54μm）上观察到芽芽增殖和繁殖的频率很高。在添加了IBA？2.46μm？的MS培养基上观察到发根，通过微繁殖产生的幼苗变硬，存活成功率为73.33％。开发的有效的微繁殖再生方案将有助于该地方性物种的非原生境保存。

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