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首页> 外文期刊>African Journal of Biotechnology >Screening and identification of cellulase producing yeast-like microorganisms from Brazilian biomes
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Screening and identification of cellulase producing yeast-like microorganisms from Brazilian biomes

机译：从巴西生物群落中筛选产生纤维素酶的酵母样微生物

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The main goals of the present study included the screening and identification of cellulase producing wild yeasts, isolated from samples collected from different Brazilian biomes. They were selected according to their capabilities of degrading carboxymethyl cellulose (CMC) and micro-crystalline cellulose (SERVACEL?), as single carbon sources in solid medium. After the step of solid medium selection, yeast cells were grown in liquid medium containing cellulose (SERVACEL?); in shake flasks at temperature of 30°C and 150?rpm agitation for 288 h. Three specific activities were evaluated: endoglucanase (CMCase), total activity (filter paper activity), and cellobiase. From a total of 390 strains of wild yeasts previously isolated, 16 strains performed cellulose hydrolysis, verified by the colorless halo in the solid medium. Among these 16 strains, 5 stood out as presenting higher levels of enzyme activity. The following step, screening in liquid medium, indicated only one strain as a potential producer of cellulases, named as AAJ6, for which the highest hydrolytic activity on carboxymethyl cellulose (0.33?U/ml) and filter paper (0.039?U/ml) was recorded. Afterwards, this wild yeast strain (AAJ6) was molecularly identified by sequencing the ITS1-5.8S-ITS2 and D1/D2 domains of the subunit (26?S) ribosomal DNA. Sequencing resulted in the identification of this strain as yeast-like fungusAcremonium strictum.
机译：本研究的主要目标包括筛选和鉴定产生纤维素酶的野生酵母，这些酵母是从巴西不同生物群落收集的样品中分离出来的。根据它们在固体培养基中作为单一碳源降解羧甲基纤维素（CMC）和微晶纤维素（SERVACEL？）的能力进行选择。固体培养基选择步骤之后，将酵母细胞在含有纤维素的液体培养基（SERVACEL 2）中生长;在摇瓶中于30°C和150？rpm搅拌下搅拌288小时。评价了三种特定的活性：内切葡聚糖酶（CMCase），总活性（滤纸活性）和纤维二糖酶。从先前分离的总共390个野生酵母菌株中，有16个菌株进行了纤维素水解，这已通过固体培养基中的无色卤素验证。在这16株菌株中，有5株表现出较高的酶活性。接下来的步骤是在液体培养基中进行筛选，结果表明只有一种菌株可能是纤维素酶的潜在生产者，称为AAJ6，其对羧甲基纤维素（0.33？U / ml）和滤纸（0.039？U / ml）的水解活性最高。被记录。然后，通过测序亚基（26ΔS）核糖体DNA的ITS1-5.8S-ITS2和D1 / D2结构域，对该野生酵母菌株（AAJ6）进行分子鉴定。测序导致该菌株被鉴定为酵母样真菌严格顶头孢霉。

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