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Application of response surface methodology to the optimization of amylase production by Aspergillus oryzae MTCC 1847

机译:响应面法在米曲霉MTCC 1847淀粉酶生产优化中的应用

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This research paper mainly focused on developing a media by optimizing parameters like sweet potato concentration, sodium nitrate concentration, pH, temperature for the maximum production of amylase by?Aspergillus oryzae?MTCC 1847. Optimization of the medium components such as sweet potato (carbon source), sodium nitrate (nitrogen source) and parameters like pH and temperature were studied for the amylase production by using response surface methodology (RSM) based central composite design (CCD). By using the surface plots and response optimizer of MINITAB 14 Software, the maximum enzyme activity of 0.893 IU/ml was predicted when sweet potato concentration was 2.01%, sodium nitrate 0.6%, pH 7.2 and temperature 25°C.
机译:该研究论文主要致力于通过优化诸如甘薯浓度,硝酸钠浓度,pH值,温度等参数来开发培养基,从而使米曲霉米曲霉MTCC 1847能够最大程度地产生淀粉酶。培养基成分的优化,例如甘薯(碳源) ),硝酸钠(氮源)以及诸如pH和温度之类的参数通过使用基于响应表面方法(RSM)的中央复合设计(CCD)研究了淀粉酶的生产。通过使用MINITAB 14 Software的表面图和响应优化器,当甘薯浓度为2.01%,硝酸钠0.6%,pH 7.2和温度25°C时,最大酶活性预计为0.893 IU / ml。

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