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首页> 外文期刊>African Journal of Biotechnology >Genetic diversity in some Ghanaian and Malian sorghum [Sorghum bicolor (L) Moench] accessions using SSR markers
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Genetic diversity in some Ghanaian and Malian sorghum [Sorghum bicolor (L) Moench] accessions using SSR markers

机译：使用SSR标记对一些加纳和马里高粱[Sorghum bicolor（L）Moench]品种的遗传多样性

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The study was carried out to assess genetic diversity among forty-one sorghum accessions obtained from Savanna Agricultural Research Institute (SARI), Nyankpala, Northern Region of Ghana and the germplasm collection of Department of Molecular Biology and Biotechnology, University of Cape Coast. Genetic diversity and relationship among the forty-one accessions were evaluated using 22 microsatellite primers. The 22 markers generated 92 alleles, with a mean of 4.2, indicating an average range of diversity. The average polymorphic information content (PIC) was 0.44, indicating that the microsatellites were informative. The cluster analysis grouped the 41 cultivars into seven distinct clusters. The most genetically distinct genotypes were Edipipii, Jibare and Belkozia, which did not cluster with any other line. The similarity between the sorghum accessions ranged from 77 to 100%. Observed heterozygosity ranged from 0 to 0.17 with an average of 0.03 per locus. Results of this study indicated that the landraces were related, and were probably exchanged between farmers in the collection regions, with some duplication found in the material, indicating that there must have been a common source of material somewhere in the history of the breeding programmes. Nonetheless, the Edipipii, Jibare and Belkozia could be exploited in breeding programmes to transfer desirable traits into elite Ghanaian sorghum cultivars.
机译：这项研究的目的是评估从加纳北部地区尼扬帕拉州萨凡纳农业研究所（SARI）获得的41个高粱品种的遗传多样性，以及开普敦海岸大学分子生物学和生物技术系的种质资源。使用22个微卫星引物评估了41个种质之间的遗传多样性和亲缘关系。 22个标记产生了92个等位基因，平均为4.2，表明多样性的平均范围。平均多态信息含量（PIC）为0.44，表明微卫星具有信息性。聚类分析将41个品种分为七个不同的聚类。遗传上最不相同的基因型是埃迪皮氏菌，吉巴尔和比尔科齐亚，它们与其他品系均不成簇。高粱种之间的相似度为77％至100％。观察到的杂合度为0至0.17，平均每个位点为0.03。这项研究的结果表明，这些地方品种是有关联的，并且可能在采集地区的农民之间进行了交换，在材料中发现了一些重复，表明在育种计划的历史上某处一定有一个共同的材料来源。但是，可以在育种计划中利用Edipipii，Jibare和Belkozia将所需的性状转移到加纳高粱品种中。

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