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首页> 外文期刊>African Journal of Biotechnology >Proteome analysis of human colorectal cancer tissue using 2-D DIGE and tandem mass spectrometry for identification of disease-related proteins
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Proteome analysis of human colorectal cancer tissue using 2-D DIGE and tandem mass spectrometry for identification of disease-related proteins

机译：使用二维DIGE和串联质谱法对人大肠癌组织进行蛋白质组分析以鉴定疾病相关蛋白

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Laser capture microdissection and two-dimensional difference gel electrophoresis were used to establish the proteomic profiles for tumor and matched adjacent tissues from 12 patients. Differential protein spots were identified by mass spectrometric analysis. The cDNA of the differential protein was transfected into colorectal cancer cells, and the biological behavior of these cells was observed. The proteomic profile in colorectal cancer tissues was significantly different from that in normal adjacent tissues. There was a 1.5-fold difference and 60 differential protein spots between cancer and adjacent tissues. Ten differential protein spots were analyzed. Among them, two protein spots were down-regulated and eight protein spots were up-regulated in the primary tumor tissues. After identification by mass spectrometry, the two down-regulated proteins were carbonic anhydrase II and protein disulfide isomerase, and these eight up-regulated proteins included APC-stimulated guanine nucleotide exchange factor, phosphoglycerate kinase 1, fumarate hydratase, aldolase A, activator protein 2B, glutathione S-transferase A3, Arginase and zinc finger protein 64 homolog. After been transfected with carbonic anhydrase II, the invasive ability, mobility and drug resistance of colon cancer lovo cells were significantly reduced. The proteomic profile was significantly different between colorectal cancer tissues and normal adjacent tissues. The down-regulation of carbonic anhydrase II and protein disulfide isomerase and up-regulation of APC-stimulated guanine nucleotide exchange facto, aldolase A, glutathione S-transferase A3 and arginase were correlated with the onset of colorectal cancer.
机译：激光捕获显微切割和二维差异凝胶电泳用于建立来自12例患者的肿瘤和匹配的相邻组织的蛋白质组学概况。通过质谱分析鉴定差异蛋白斑点。将该差异蛋白的cDNA转染到结直肠癌细胞中，并观察了这些细胞的生物学行为。大肠癌组织中的蛋白质组学特征与正常邻近组织中的蛋白质组学特征显着不同。癌与邻近组织之间存在1.5倍的差异和60个差异蛋白质点。分析了十个差异蛋白斑点。其中，在原发性肿瘤组织中两个蛋白点被下调，八个蛋白点被上调。经质谱鉴定后，两个被下调的蛋白分别为碳酸酐酶II和二硫键异构酶，这八个被上调的蛋白包括APC刺激的鸟嘌呤核苷酸交换因子，磷酸甘油酸激酶1，富马酸水合酶，醛缩酶A，激活蛋白2B。 ，谷胱甘肽S-转移酶A3，精氨酸酶和锌指蛋白64同源物。用碳酸酐酶II转染后，结肠癌Lovo细胞的侵袭能力，迁移率和耐药性显着降低。结直肠癌组织和正常邻近组织之间的蛋白质组学差异显着。碳酸酐酶II和蛋白质二硫键异构酶的下调以及APC刺激的鸟嘌呤核苷酸交换因子，醛缩酶A，谷胱甘肽S-转移酶A3和精氨酸酶的上调与大肠癌的发生有关。

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