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首页> 外文期刊>African Journal of Biotechnology >Repeated-batch ethanol fermentation from sweet sorghum juice by free cells of Saccharomyces cerevisiae NP 01
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Repeated-batch ethanol fermentation from sweet sorghum juice by free cells of Saccharomyces cerevisiae NP 01

机译：酿酒酵母NP 01从游离高粱汁中分批重复发酵乙醇

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摘要

Low-cost inoculum preparation (IP) media for ethanol production were developed. It was found that sweet sorghum juice (SSJ) containing 100 g l-1?of total sugar without nutrient supplement could be used as the low-cost IP medium instead of the typical IP medium or yeast extract malt extract (YM) medium. Ethanol production from the SSJ (total soluble solids of 24 °Bx) by the inoculum of?Saccharomyces cerevisiaeNP 01 in batch and repeated-batch fermentations was then investigated. The fermentations were carried out under static condition in 500 ml air-locked Erlenmeyer flasks at 30°C and the initial yeast cell concentration was 1×108?cells ml-1. In the batch fermentation, the concentration (P), productivity (Qp) and yield (Yp/s) of ethanol were 110.09 ± 0.81 g l-1, 2.29 ± 0.01 g l-1?h?-1?and 0.51 ± 0.02, respectively. In the repeated-batch fermentation, the yeasts could be used at least eight successive batches without a marked decrease in ethanol production. The repeated-batch fermentation with fill and drain volume at 75% of the working volume gave higher Ethanol production efficiencies than those at 50% of the working volume in terms of total Ethanol production rate (g h-1). The average?P,?Qp?and?Yp/sof the eight successive batches at 75% fill and drain volume in a 2 L bioreactor at the agitation rate of 100 rev min-1?were 93.30 ± 9.44 g l-1, 1.21 ± 0.43 g l-1?h?-1?and 0.48 ± 0.03, respectively.
机译：开发了用于乙醇生产的低成本接种物（IP）培养基。已经发现，包含100 g l-1？总糖而不添加营养补充剂的甜高粱汁（SSJ）可以用作低成本IP培养基，而不是典型的IP培养基或酵母提取物麦芽提取物（YM）培养基。然后，研究了酿酒酵母NP 01的分批和重复分批发酵从SSJ（总可溶性固体含量为24°Bx）产生乙醇的过程​​。发酵在静态条件下在500 ml气锁锥形瓶中于30°C进行，初始酵母细胞浓度为1×108？cells ml-1。在分批发酵中，乙醇的浓度（P），生产率（Qp）和产率（Yp / s）分别为110.09±0.81 g l-1、2.29±0.01 g l-1？h？-1？和0.51±0.02 ， 分别。在重复分批发酵中，酵母至少可以连续使用八批，而乙醇的产量却没有明显下降。就总乙醇生产率（g h-1）而言，填充和排出体积为工作体积的75％的重复分批发酵比在50％工作体积的情况下的乙醇生产效率更高。在100升min-1的搅拌速率下，在2 L生物反应器中填充和排出量为75％的情况下，八个连续批次的平均P，Qp和Yp / s为93.30±9.44 g l-1，1.21分别为±0.43g l-1？h？-1？和0.48±0.03。

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