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首页> 外文期刊>African Journal of Biotechnology >Assessment and characterization of genetic diversity in Withania somnifera (L.) Dunal using RAPD and AFLP markers
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Assessment and characterization of genetic diversity in Withania somnifera (L.) Dunal using RAPD and AFLP markers

机译：利用RAPD和AFLP标记评估和评估催眠催花（Withania somnifera（L.）Dunal）的遗传多样性

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Genetic diversity of 23 accessions of?Withania somnifera?collected from different geographical regions of India was estimated by employing Random Amplification of Polymorphic DNA (RAPD) and Amplified Fragment Length Polymorphism (AFLP) markers. Eighteen RAPD primers and six AFLP primer combinations revealed 37.82 and 43.94% polymorphism, respectively, among 163 and 286 genetic loci amplified. The AFLP assay revealed higher levels of polymorphism among the tested?W. somnifera?accessions compared to the RAPD. Mean genetic diversity based on Shannon index ranged from 1.33 (RAPD) to 5.13 (AFLP). Unweighted pair group method based on arithmetic average (UPGMA) analysis was performed on Jaccard’s similarity coefficient matrix. The matrix reveals, two main clusters, wild accessions formed one cluster and the cultivated accessions formed the other. The cultivated accessions are well separated from the wild ones at a low similarity value of 0.3, indicating that cultivated and wild accessions are highly distinct. Morphologically cultivated accessions were also quite distinct from the wild ones and the cluster analysis of RAPD and AFLP fingerprints clearly discriminated the five cultivated accessions of?W. somnifera. A strong correlation was observed between morphology and molecular marker systems. Identification of specific markers to wild as well as cultivated accessions is yet another important finding in the present study. Such genetic diversity is useful in facilitating the development of large number of new varieties through hybridization, transfer of useful genes, thus maximizing the use of such available germplasms as genetic resource materials for breeders. The present input, first of its kind in Ashwagandha, will thus assist the marker assisted crop improvement programme.
机译：采用多态性DNA随机扩增（RAPD）和片段长度多态性扩增（AFLP）标记，对印度不同地区的23个冬枯萎病菌的遗传多样性进行了估算。在扩增出的163和286个遗传基因座中，有18种RAPD引物和6种AFLP引物组合分别显示出37.82和43.94％的多态性。 AFLP分析显示被测ΔW中较高的多态性水平。与RAPD相比，Somnifera？种质。基于香农指数的平均遗传多样性范围为1.33（RAPD）至5.13（AFLP）。在Jaccard的相似系数矩阵上执行了基于算术平均值（UPGMA）分析的非加权对组方法。矩阵揭示了两个主要簇，野生种形成一个簇，而栽培种形成另一个簇。栽培种与野生种的分离度很低，相似度为0.3，表明栽培种和野生种的差异很大。形态培养的种质也与野生种完全不同，RAPD和AFLP指纹图谱的聚类分析清楚地区分了5个栽培种的W。茄子在形态学和分子标记系统之间观察到强烈的相关性。鉴定野生和栽培种的特定标记是本研究中的另一个重要发现。这种遗传多样性有助于通过杂交，有用基因的转移促进大量新品种的开发，从而最大限度地利用此类可用种质作为育种者的遗传资源。因此，目前在Ashwagandha进行的此类投入将有助于标志物辅助作物改良计划。

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