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首页> 外文期刊>African Journal of Biotechnology >Embryogenesis of Gentiana straminea and assessment of genetic stability of regenerated plants using inter simple sequence repeat (ISSR) marker
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Embryogenesis of Gentiana straminea and assessment of genetic stability of regenerated plants using inter simple sequence repeat (ISSR) marker

机译：利用简单序列间重复序列（ISSR）标记，秦ent的胚发生和再生植物的遗传稳定性评估

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Gentiana straminea?Maxim is an endangered medicinal plant in the Qinghai-Tibet Plateau. To speed up the production of this species, an?in vitro?protocol for efficient plant regeneration was developed from its leaf explants. Auxins and cytokinin alone or in combination were examined for their effects on callus induction and plant regeneration. The results indicated that Murashige and Skoog medium supplemented with 2 mg l-1?2,4-dichlorophenoxyacetic acid and 0.5 mg l-1?N6-benzylaminopurine (BA) was the best medium for embryogenic callus induction, while 3 mg l-1?BA induced the highest frequency (93.8%) of regeneration and development of shoots. Regenerated plantlets with well-developed root systems were?hardened in the greenhouse and successfully established in the soil. Genetic stability of the regenerated plants was assessed by 25?inter simple sequence repeat?(ISSR) markers. Out of 25 ISSR markers, 14 produced clear, reproducible bands with a mean of 6.9 bands per marker. The results confirmed that the regenerants maintained high genetic fidelity.
机译：Gentiana straminea？Maxim是青藏高原的一种濒危药用植物。为了加快该物种的生产，从其叶片外植体开发了一种有效植物再生的“体外”协议。检查生长素和细胞分裂素的单独或组合对愈伤组织诱导和植物再生的影响。结果表明，补充2 mg l-1？2,4-二氯苯氧基乙酸和0.5 mg l-1？N6-苄基氨基嘌呤（BA）的Murashige和Skoog培养基是诱导胚性愈伤组织的最佳培养基，而3 mg l-1 ？BA诱导了芽的再生和发育的最高频率（93.8％）。根系发达的再生小植株在温室中硬化，并在土壤中成功建立。通过25？inter简单序列重复？（ISSR）标记评估再生植株的遗传稳定性。在25个ISSR标记中，有14个产生清晰，可复制的条带，每个标记平均6.9条带。结果证实了再生体保持了高遗传保真度。

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