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首页> 外文期刊>African Journal of Biotechnology >Development of efficient micropropagation protocol for Withania coagulans (Stocks) Dunal
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Development of efficient micropropagation protocol for Withania coagulans (Stocks) Dunal

机译：开发有效的微繁殖方案，用于Withania凝聚物（股票）杜纳尔

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The present study describes the development of efficient micropropagation protocol for a commercially important medicinal plant?Withania coagulans.?Nodal segments were immersed in various concentrations of cefotaxime, viz 100, 250, 500 and 750 mgl-1?for 5 min and implanted on Murashige and Skoog medium (MS) medium fortified with 6-benzyladenine (BA) (2 to 4 mgl-1) or indolebutyric acid (IBA) (0.25 to 0.5 mgl-1) either alone or in combination with different concentrations. Of the four concentrations of cefotaxime tested, the 250 mgL-1?showed nonphytotoxic effect on cultures and completely eliminated bacterial infection. Direct multiple shoots differentiation occurred in cultured explants without intervening callus phase and the maximum number of shoots (7.2 ± 1.0 per explant) and elongation (7 ± 1.4 cm) were achieved on MS media containing 2 mgl-1?BA + 0.5 mgl-1?IBA. For induction of stout root system, the shoot buds were cultured on ?MS medium fortified with different concentrations of IBA (1 to 4 mgl-1), indole-3-acetic acid (IAA) (0.25 to 1.0 mgl-1) and kinetin (Kin) (1 to 2 mgl-1). MS medium with 2 mgl-1?IBA was found most effective for the induction of stout root system. Well-rooted plantlets were transferred to outside pots containing sterile soil, and sand mixture (2:1) showed 75% survival.
机译：本研究描述了一种有效的微繁殖方案的发展，该方案用于商业上重要的药用植物“ Withania凝结素”。将节段浸入不同浓度的头孢噻肟中，即100、250、500和750 mgl-1？5分钟，然后植入Murashige。以及单独或组合使用不同浓度的6-苄腺嘌呤（BA）（2至4 mgl-1）或吲哚丁酸（IBA）（0.25至0.5 mgl-1）强化的Skoog培养基（MS）。在测试的四种浓度的头孢噻肟中，250 mgL-1？对培养物显示出非植物毒性作用，并完全消除了细菌感染。培养的外植体中直接发生了多芽分化，而没有中间的愈伤组织相，在含有2 mgl-1？BA + 0.5 mgl-1的MS培养基上达到了最大芽数（每个外植体7.2±1.0）和伸长（7±1.4 cm）。 ？IBA。为了诱导粗壮的根系，将芽芽在？MS培养基上培养，该培养基用不同浓度的IBA（1-4 mgl-1），吲哚-3-乙酸（IAA）（0.25-1.0 mgl-1）和激动素（Kin）（1至2 mgl-1）。发现具有2 mgl-1？IBA的MS培养基对诱导粗壮的根系最有效。根深蒂固的小植株转移到装有无菌土壤的室外盆栽中，沙混合物（2：1）显示75％的存活率。

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