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首页> 外文期刊>African Journal of Biotechnology >Micropropagation of Ocotea porosa (Nees Martius) Barroso
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Micropropagation of Ocotea porosa (Nees Martius) Barroso

机译：Ocotea porosa（Nees Martius）Barroso的微繁殖

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The objective of this work was the establishment of a micropropagation protocol forOcotea porosa?by multiplication of shoots from axillary buds. Different concentration of BAP (0; 2.5; 5 or 10 μM) or BAP+KIN (0; 1.25; 2.5 or 5 μM) were investigated to optimize the multiplication. Shoot growth was stimulated with reduced concentration of BAP (0; 0.5; 1 or 1.5 μM) or KIN (0.5 or 1μM) or activated charcoal (0.5; 1; 2 or 3 gl-1). For root induction different concentrations of IBA (0; 1.25; 2.5; 5 or 10 μM) or (0; 2.5; 5 or 10 mM) were applied. The highest mean multiplication rate was observed in the fourth subculture with 5 μM BAP, reaching 5.3 shoots per explant. The shoots elongated in culture medium supplemented with 2 gl-1?activated charcoal and presented bigger leaves than on medium with reduced concentration of BAP. The shoots rooted on medium contains 10 μM IBA or after pulse treatment of 10 mM (68.7 and 62.6% of rooting, respectively). The survival rate of the plants was 56.7%. This study showed that?O. porosa?micropropagation is feasible; however it needs further research in order to increase plant survival.
机译：这项工作的目的是通过繁殖来自腋芽的芽来建立一种用于Ocotea porosa？的微繁殖方案。研究了不同浓度的BAP（0; 2.5; 5或10μM）或BAP + KIN（0; 1.25; 2.5或5μM）以优化乘法。 BAP（0; 0.5; 1或1.5μM）或KIN（0.5或1μM）或活性炭（0.5; 1; 2或3 gl-1）浓度降低可刺激枝条生长。对于根诱导，应用不同浓度的IBA（0; 1.25; 2.5; 5或10μM）或（0; 2.5; 5或10 mM）。在使用5μMBAP的第四次传代培养中观察到最高平均繁殖率，每个外植体达到5.3枝。在补充有2 gl-1？活性炭的培养基中，枝条伸长，且叶片上的芽比在BAP浓度降低的培养基上长。生根在培养基上的芽含有10μMIBA或经过10 mM的脉冲处理（分别为生根的68.7和62.6％）。植物的成活率为56.7％。这项研究表明？多孔微繁殖是可行的;然而，为了增加植物的存活率，还需要进一步的研究。

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