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首页> 外文期刊>Revista rvore >Germina??o in vitro de eixos embrionários zigóticos de imbuia (ocotea porosa (NEES EX MARTIUS) Liberato Barroso)
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Germina??o in vitro de eixos embrionários zigóticos de imbuia (ocotea porosa (NEES EX MARTIUS) Liberato Barroso)

机译：植物胚胎Zigothal轴的体外萌发（Octea Porosa（Nees Ex Martius）解释了Barroso）

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摘要

Imbuia has recalcitrant seeds with strong tegumental dormancy, irregular and low germination, which impair natural propagation. The objective of this study was to evaluate the in vitro germination of imbuia embryonic axes by testing the effect of different concentrations of sucrose, activated charcoal and salt formulations in order to accelerate the production of plants. Zygotic embryonic axes were inoculated in MS/2 culture medium with different concentrations of sucrose (15, 30, 60 or 90 g L-1), of activated charcoal (0, 1, 2 or 3 gL-1) and in different salt formulations (MS, WPM, MS/2 or WPM/2). The results indicated that the addition of 15 g L-1 sucrose was not enough to promote germination of embryonic axes and the other concentrations promoted between 45.5 and 59.4% germination. The best results were observed with MS and MS/2 salt formulations obtained 67.7 and 74.2% of germination respectively. The addition of activated charcoal to the media favored the in vitro germination of zygotic embryonic axes, while oxidation of the embryo tissues occurred in media without activated charcoal and germination did not happen. The in vitro germination of imbuia can be achieved with the MS/2 salt formulation supplemented with 30 g L-1 sucrose and 1 g L-1 of activated charcoal.
机译：胰岛素具有顽固的种子，具有较强的Tegumental休眠，不规则和低萌发，损害自然繁殖。本研究的目的是通过测试不同浓度蔗糖，活性炭和盐配方的效果来评估胰岛胚轴轴的体外萌发，以加速植物的生产。在具有不同浓度的蔗糖（15,30,60或90g L-1）的MS / 2培养基中接种Zygotic胚胎轴，活性炭（0,1,2或3gl-1）和不同的盐配方（MS，WPM，MS / 2或WPM / 2）。结果表明，加入15g L-1蔗糖不能促进胚胎轴的发芽，另一种浓度促进在45.5％和59.4％的萌发中。使用MS和MS / 2盐制剂分别观察到最佳结果67.7和74.2％的发芽。向培养基中加入活性炭有利于促狗胚胎轴的体外萌发，而在没有活化的木炭的培养基中发生胚胎组织的氧化，不会发生发芽。通过补充有30g L-1蔗糖的MS / 2盐制剂和1g L-1的活性炭，可以实现胰腺的体外萌发。

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