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首页> 外文期刊>African Journal of Biotechnology >Medium optimization for endochitinase production by recombinant Pichia pastoris ZJGSU02 using response surface methodology
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Medium optimization for endochitinase production by recombinant Pichia pastoris ZJGSU02 using response surface methodology

机译：使用响应面法优化重组巴斯德毕赤酵母ZJGSU02生产内切几丁质酶的培养基

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摘要

Plackett-Burman design and response surface methodology were employed to optimize the medium components for endochitinase production by?Pichia pastoris. A Plackett- Burman design of seven factors with 12 runs was applied to evaluate the effects of different medium components. Yeast extract, oleic acid and Tween-80 were found to have significant influence on endochitinase production. The optimal concentrations of three factors were investigated by the response surface methodology using Box- Behnken design. The optimal medium components obtained for achieving the maximum activity of the endochitinase were as follows: Yeast extract 24.36 g/l, tryptone 20 g/l, YNB 5.0 g/l, potassium phosphate 100 mM, methanol 5 ml/l, oleic acid 1.758 ml/l, Tween-80 6.2 ml/l, Pichia trace metals (PTM1) 4.0 ml/ l and biotin 4.00 × 10-4?g/l. Under these conditions, endochitinase activity was up to 88.26 μ/ml, which was about 1.14-fold higher than using the original medium (77.62 μ/ml). This work will be very helpful for large-scale production of endochitinase for future industrial application.
机译：采用Plackett-Burman设计和响应面方法优化巴斯德毕赤酵母生产内切酶的培养基成分。采用Plackett-Burman设计的7个因子进行12次分析，以评估不同培养基成分的影响。发现酵母提取物，油酸和Tween-80对内切几丁质酶的产生有重要影响。使用Box-Behnken设计，通过响应面方法研究了三个因素的最佳浓度。为实现内切几丁质酶的最大活性而获得的最佳培养基成分如下：酵母提取物24.36 g / l，胰蛋白20 20 g / l，YNB 5.0 g / l，磷酸钾100 mM，甲醇5 ml / l，油酸1.758 ml / l，Tween-80 6.2 ml / l，毕赤酵母微量金属（PTM1）4.0 ml / l和生物素4.00×10-4？g / l。在这些条件下，内切几丁质酶活性高达88.26μ/ ml，比使用原始培养基（77.62μ/ ml）高约1.14倍。这项工作对于将来的工业应用大规模生产内切壳多糖酶将非常有帮助。

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