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首页> 外文期刊>African Journal of Biotechnology >Differential protein expression in maize (Zea mays) in response to insect attack
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Differential protein expression in maize (Zea mays) in response to insect attack

机译：玉米（Zea mays）对昆虫侵袭的差异蛋白表达

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摘要

Maize (Zea mays) is a major food stable in sub-Saharan Africa. However, yields are constrained by insect pests. Insect feeding induces a number of changes in genes encoding different proteins and the plant’s response can either be direct or indirect, or both. In this study,?maize plants were infested with two insects with different feeding strategies (Spodoptera littoralis, chewing insect and?Busseola fusca, stem borer) to investigate differential protein expression using the Proteomics technique. Infestation of?S. littoralis?(3rd instar larvae) resulted in 14 spots being up-regulated and 7 being down-regulated. Similarly,?infestation of maize with?B. fusca?(3rd instar larvae) resulted in 12 spots being up-regulated and 9 spots being down-regulated. Interestingly, of those up-regulated only 9 were common to both insects, with only 4 common to both in terms of down regulation. Infestation of maize with?S. littoralisresulted in a greater number of spots being up-regulated and less being down-regulated compared to maize infested with?B. fusca. Unfortunately,?we were unable to identify the proteins represented by these spots.
机译：玉米（Zea mays）是撒哈拉以南非洲的主要粮食稳定地。但是，产量受到虫害的限制。昆虫摄食会导致编码不同蛋白质的基因发生许多变化，植物的反应可以是直接的或间接的，或两者都有。在这项研究中，玉米植株感染了两种昆虫，它们具有不同的饲养策略（斜纹夜蛾，咀嚼昆虫和Bu蒲、,虫），以利用蛋白质组学技术研究蛋白质的差异表达。 S。 littoralis？（三龄幼虫）导致14个斑点上调，而7个斑点下调。同样，玉米对B的侵染。 fusca？（三龄幼虫）导致上调12个斑点，下调9个斑点。有趣的是，在上调的那些中，只有两种昆虫共有9种，而在下调方面，只有两种是共有的。玉米侵染？与用？B侵染的玉米相比，沿海地区导致大量斑点被上调而下调较少。福斯卡。不幸的是，我们无法鉴定这些斑点代表的蛋白质。

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