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Toxicity of local Malaysian Bacillus thuringiensis subspecies Kurstaki against Plutella xylostella

机译:马来西亚苏云金芽孢杆菌亚种Kurstaki对小菜蛾的毒性

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The toxicity effect of?Bacillus thuringiensis?against?Plutella xylostella?is well established. However, effective?B. thuringiensis?strain especially local isolate is not well tested. In this study local strain?B. thuringiensis?subspecies?kurstaki, SN5 was assessed for its effectiveness against?P. xylostella?3rd instar larvae. Other factors such as spore germination, spore coat, L-alanine-adenosine (LAA) and streptomycin were evaluated with their possible effects on the toxicity of?B. thuringiensis?cry protein. The result of the study showed that SN5 spore exhibit higher toxicity than the commercial strain, HD-1. L-Alanine-adenosine not only improves rate of spore germination but also synergy effect of spore-crystal mix by increasing toxicity of the mixture. These results demonstrating the potential of local isolate in managing?P. xylostella?and its potential effect can be increase by adding LAA.
机译:苏云金芽孢杆菌对小菜蛾的毒性作用已得到充分证实。但是有效吗?苏云金芽孢杆菌菌株,特别是本地分离株尚未得到很好的测试。在这项研究中,本地菌株?苏云金芽孢杆菌亚种kurstaki,SN5评估了其对?P的有效性。 xylostella?三龄幼虫。评估了其他因素,如孢子萌发,孢子皮,L-丙氨酸-腺苷(LAA)和链霉素,它们对βB的毒性可能具有影响。苏云金芽孢杆菌哭泣蛋白。研究结果表明,SN5孢子比商业菌株HD-1具有更高的毒性。 L-丙氨酸-腺苷不仅提高了孢子发芽率,而且通过增加混合物的毒性提高了孢子-晶体混合物的协同作用。这些结果证明了局部隔离在管理P方面的潜力。通过添加LAA可以增加木聚糖酶及其潜在作用。

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