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首页> 外文期刊>African Journal of Biotechnology >Functional analysis of a gene encoding homoserine kinase from rice
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Functional analysis of a gene encoding homoserine kinase from rice

机译：水稻高丝氨酸激酶编码基因的功能分析

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Homoserine kinase (HSK) is an enzyme that catalyzes the common pathway in threonine and methionine biosynthesis in plants. The genes encoding HSK have been reported in many bacteria and some plants including Arabidopsis. Our group recently reported a gene for threonine synthase (TS) from rice. In this study, we functionally characterized a gene encoding HSK from rice (OsHSK). Analysis of a cDNA sequence and genome of rice revealed that a full-length open leading frame of OsHSK consisted of 378 amino acids, which corresponded to a protein with the molecular weight of approximately 37.8 kDa and with the predicted isoelectric point of 6.86. The predicted amino acid sequence of OsHSK harbored a distinct signature motif for ATP binding and was highly homologous to that of enzymes of plant and bacterial HSKs. Expression of OsHSK in the?thrB?mutant of?Escherichia coli?showed that the gene was able to functionally complement the mutant. These results suggest that OsHSK encodes a protein for HSK in rice.
机译：同型丝氨酸激酶（HSK）是一种催化植物苏氨酸和蛋氨酸生物合成中共同途径的酶。已经在许多细菌和一些植物包括拟南芥中报道了编码HSK的基因。我们的小组最近报道了水稻中的苏氨酸合酶（TS）基因。在这项研究中，我们在功能上表征了水稻中编码HSK的基因（OsHSK）。水稻cDNA序列和基因组的分析表明，OsHSK的全长开放前导框架由378个氨基酸组成，对应于分子量约为37.8 kDa的蛋白质，预测的等电点为6.86。 OsHSK的预测氨基酸序列具有与ATP结合的独特特征基序，与植物和细菌HSK的酶高度同源。 OsHSK在大肠杆菌“ thrB”突变体中的表达表明该基因能够在功能上与该突变体互补。这些结果表明，OsHSK编码水稻中HSK的蛋白质。

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