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首页> 外文期刊>African Journal of Biotechnology >Isolation, genetic diversity and identification of a virulent pathogen of eriophyid mite, Aceria guerreronis (Acari: Eriophyidae) by DNA marker in Karnataka, India
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Isolation, genetic diversity and identification of a virulent pathogen of eriophyid mite, Aceria guerreronis (Acari: Eriophyidae) by DNA marker in Karnataka, India

机译：DNA标记在印度卡纳塔克邦分离，遗传多样性和毒气螨，埃塞俄比亚游击队（Acari：绒毛纲）的致病性病原体的鉴定。

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Aceria guerreronis?is a serious pest of coconut in India. Investigations were carried out to investigate fungal pathogens infecting the eriophyid mites for their utilisation as biocontrol agents in Karnataka, India. The fungal pathogens namely,?Hirsutella thompsonii,?Beauveria bassiana,?Fusarium semitectum?and few opportunistic pathogens namely,?Fusarium moniliforme,?Cladosporium tennuissimum,?Aspergillus niger,?Penicillium?sp. and?Mucor?sp. were collected from eriophyid mite populations in different parts of Karnataka area. Of the total collected nuts, 3.54% were infected byH. thompsonii,?2.46 and 0.29% by?B. bassiana?and?F. semitectum,?respectively. A lower number of nuts (0.03 to 0.79%) were infected by opportunistic pathogens. The incidence of pathogen infected coconuts in areas with lower temperature and higher humidity were ranged from 4.37 to 19.52% whereas with higher temperature and lower humidity it was 0 to 4.54%. Occurrence of?B. bassiana?and?F. semitectum?on?A. guerreronis?are new records. Among isolates of?H. thompsonii?collected from different places, the isolate Bangalore?was more virulent followed by Mysore, Mandya, Kanakapura, Arsikere and Hiriyur isolates, as it recorded maximum infection (HTCMBAN- 88.63%). The?B. bassiana?isolates caused mortality ranging from 72.87 to 86.97%. The virulent isolate?H. thompsonii?(HTCMBAN) was tested at different concentrations, with increase in the concentration mortality rate. The genetic diversity of isolates of?H. thompsonii?by random amplification of polymorphic DNA (RAPD) revealed that grouping of the isolates was in accordance with geographic location. DNA fragments of 850 and 950 bp (OPA-20) were specific to the virulent isolate HTCMBAN.
机译：Aceria guerreronis？是印度的一种严重的椰子害虫。在印度卡纳塔克邦，进行了调查以调查感染线虫螨的真菌病原体作为生物防治剂。真菌病原体为“ Hirsutella thompsonii”，“ Beauveria bassiana”，“ Fusarium semitectum”和少量机会病原体，即“ Fusarium moniliforme”，“ Cladosporium tennuissimum”，“ Aspergillus niger”，“ Penicillium” sp。和？Mucor？sp。从卡纳塔克邦不同地区的螨类螨种群中收集。在收集的所有坚果中，有3.54％被H感染。汤普森（B）分别为2.46和0.29％。低音提琴和？ Semitectum，分别。机会性病原体感染了较少数量的坚果（0.03至0.79％）。在较低温度和较高湿度的地区，被病原体感染的椰子的发生率在4.37％至19.52％之间，而在较高温度和较低湿度的情况下，其发生率为0至4.54％。 B．低音提琴和？ Semitectum？on？ guerreronis？是新记录。在？H的分离株中。从不同地方收集的thompsonii分离株更具毒性，其次是Mysore，Mandya，Kanakapura，Arsikere和Hiriyur分离株，因为它记录了最大的感染（HTCMBAN- 88.63％）。 B？鲈鱼分离物引起的死亡率在72.87至86.97％之间。强毒分离株？在不同浓度下测试了thompsonii？（HTCMBAN），随着浓度死亡率的增加。 H.H.分离株的遗传多样性。汤普森氏菌通过随机扩增多态性DNA（RAPD）揭示，分离株的分组与地理位置一致。 850和​​950 bp（OPA-20）的DNA片段对强毒分离株HTCMBAN具有特异性。

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