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首页> 外文期刊>Cord >Genetic diversity analysis of coconut eriophyid mite (Aceria guerreronis Keifer) (Acari: Eriophyidae) population based on inter simple sequence repeat (ISSR) markers
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Genetic diversity analysis of coconut eriophyid mite (Aceria guerreronis Keifer) (Acari: Eriophyidae) population based on inter simple sequence repeat (ISSR) markers

机译:基于内部简单重复序列(ISSR)标记的椰子螨(Aceria guerreronis Keifer)(Acari:Eriophyidae)种群的遗传多样性分析

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摘要

DNA markers have been employed on several insect species to estimate the genetic diversity across their populations. In the present study, an attempt was made to survey the extent of diversity among the coconut eriophyid mite [Aceria guerreronis Keifer Eriophyidae: Acari] populations collected from various ecological zones of Tamil Nadu, India. A total of 13 samples of eriophyid mite were analyzed by using 5 inter simple sequence repeat (ISSR) primers. The number of scorable markers produced by an individual primer ranged from 3-20 with an average of 10.6 markers per primer. Out of 53 markers generated with 5 ISSR primers, 11 were polymorphic across the 13 mite samples with an overall polymorphism of 20.75%. The lowest and the highest level of polymorphism were observed with primers viz. ISSR02 (30.76%) and ISSR01 (100%), respectively. The data matrix obtained for the presence or absence of markers across the 13 mite populations was analyzed to estimate the genetic distance between each sample. Theanalysis indicated that the mite populations collected from different locations are almost similar, genetically.
机译:DNA标记已用于几种昆虫,以估计其种群之间的遗传多样性。在本研究中,我们尝试调查了从印度泰米尔纳德邦各个生态区收集的椰子盐藻螨(Aceria guerreronis Keifer Eriophyidae:Acari)种群之间的多样性程度。通过使用5种内部简单序列重复(ISSR)引物分析了总共13个螨样品。单个引物产生的可评分标记数范围为3-20,每个引物平均为10.6。用5个ISSR引物生成的53个标记中,有11个在13个螨样品中具有多态性,总体多态性为20.75%。用引物viz观察到最低和最高水平的多态性。 ISSR02(30.76%)和ISSR01(100%)。分析获得的13个螨虫种群中是否存在标记物的数据矩阵,以估计每个样品之​​间的遗传距离。分析表明,从遗传角度来看,从不同地点收集的螨类种群几乎相似。

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