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首页> 外文期刊>African Journal of Biotechnology >Isolation of a novel MYB transcription factor OsMyb1R from rice and analysis of the response of this gene to abiotic stresses
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Isolation of a novel MYB transcription factor OsMyb1R from rice and analysis of the response of this gene to abiotic stresses

机译：从水稻中分离出一种新型MYB转录因子OsMyb1R，并对该基因对非生物胁迫的响应进行了分析

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To understand mechanisms underlying stress responses and discover new stress-tolerance genes in rice (Oryza sativa?L.), we analyzed a global genome expression profiling of the?indica?cultivar Pei’ai 64S subjected to cold, drought, or heat stresses. Expression profiles were obtained for leaf and panicle tissues at seedling, booting and heading stages from plants under no stress, or cold, drought or heat stresses using the GeneChip Rice Genome Array (Affymetrix) representing 51 279 transcripts fromjaponica?and?indica?rice. A large number of genes highly up- regulated or down-regulated were identified under the stresses. One of these genes,?OsMyb1R,?was highly induced in leaf and panicle at the heading and flowering stages, in response to all stresses. The expression profile of?OsMyb1R?obtained by the microarray analysis was confirmed by quantitative real-time?reverse transcriptase-plolymerase chain reaction (RT-PCR)?analysis of the gene. The two sets of data matched very well, suggesting that?OsMyb1R?is a multiple stresses responsive gene in rice. In order to study its function in stress tolerance, we cloned the cDNA of the gene through amplification by RT-PCR. Sequence analysis showed that the cDNA encodes a protein of 489 amino acid residues with molecular weight (M.W.)?≈?56 kD and pI?≈ 6. Bioinformatics analysis revealed that the protein contains a conservative domain ?of the MYB family gene. Analysis of the putative promoter region for candidate?cis-regulatory elements identified 5 matches to?cis-elements related to stress responses. Based on the earlier mentioned analysis and results obtained, we propose thatOsMyb1R?is a novel candidate gene involved in stress tolerance in rice.
机译：为了了解潜在的胁迫反应机制并发现水稻（Oryza sativa？L。）中新的耐胁迫基因，我们分析了低温，干旱或高温胁迫下印度indPeiai 64S的全球基因组表达谱。使用GeneChip Rice Genome Array（Affymetrix），代表了来自粳稻和ind稻的51 279个转录本，从无胁迫，冷，干旱或热胁迫下的植物获得了幼苗，启动和抽穗期叶片和穗组织的表达谱。在压力下鉴定出大量上调或下调的基因。这些基因之一，“ OsMyb1R”，在抽穗期和开花期对叶片和圆锥花序产生高响应，以应对所有胁迫。通过基因的实时定量逆转录酶-聚合酶链反应（RT-PCR）分析证实了通过微阵列分析获得的“ OsMyb1R”的表达谱。两组数据非常吻合，表明“ OsMyb1R”是水稻中的多重应激反应基因。为了研究其在胁迫耐受性中的功能，我们通过RT-PCR扩增来克隆该基因的cDNA。序列分析表明该cDNA编码具有489个氨基酸残基的蛋白质，其分子量（M.W.）≥≈56kD，pI≥≈6。生物信息学分析表明该蛋白质含有一个MYB家族基因的保守结构域。分析候选启动子区域的顺式调控元件，发现与应激反应相关的顺式元件有5个匹配。根据前面提到的分析和获得的结果，我们认为OsMyb1Rα是一种参与水稻耐逆性的新候选基因。

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