首页> 外文期刊>African Journal of Biotechnology >Genetic variation among Iranian oaks (Quercus spp.) using random amplified polymorphic DNA (RAPD) markers
【24h】

Genetic variation among Iranian oaks (Quercus spp.) using random amplified polymorphic DNA (RAPD) markers

机译:利用随机扩增多态性DNA(RAPD)标记对伊朗栎(Quercus spp。)之间的遗传变异

获取原文
           

摘要

Quercus?is one of the most important woody genera of the Northern hemisphere and considered as one of the main forest tree species in Iran. In this study, genetic relationships in the genus?Quercus, using random amplified polymorphic DNA (RAPD) was examined. Five species, including:?Quercus robur, Quercus macranthera, Quercus infectoria, Quercus magnosquamata?and?Quercus libani?were collected from Northwest forests of Iran and analyzed. Each tree was judged as a genuine type of each species according to the morphological structures. 10 RAPD primers reproducibly and strongly generated 104 discrete markers, ranging from 100 to 3000 bp. 100 of these were polymorphic (96%), with an average of 10.4 markers per primer. Jaccard`s similarity coefficient was calculated, and a dendrogram constructed, based on unweighted pair group method analysis (UPGMA). Cluster analysis of RAPD data, using the NTSYSpc 2.02 resulted in two clusters. Genetic analysis represented relatively high interspecific genetic distance, ranging from a minimum of 0.298 between?Q. libani?and?Q. magnosquamata?to a maximum of 0.625 between?Q. macranthera?and?Q. magnosquamata. The range in distance coefficient indicated high variation among species for?Quercus?genus in Northwest forests of Iran. This result suggests that RAPD marker is useful for?Quercus?genetic diversity analysis.
机译:栎属是北半球最重要的木属之一,被认为是伊朗主要的林木树种之一。在这项研究中,使用随机扩增多态性DNA(RAPD)研究了栎属的遗传关系。从伊朗西北森林中收集了五种物种,包括:长白栎(Quercus robur),白栎(Quercus macranthera),栎属(Quercus infectoria),大白栎(Quercus magnosquamata)和白栎(Quercus libani)。根据形态结构,将每棵树判断为每个物种的真实类型。 10种RAPD引物可重现并强烈生成104个离散标记,范围从100到3000 bp。其中100个是多态性的(96%),每个引物平均带有10.4个标记。基于未加权对组方法分析(UPGMA),计算了Jaccard的相似系数,并构建了树状图。使用NTSYSpc 2.02对RAPD数据进行聚类分析得到两个聚类。遗传分析表示相对较高的种间遗传距离,介于QQ之间的最小0.298。问: Q值之间最大为0.625。 Macranthera和Q.木兰。距离系数的范围表明伊朗西北森林栎类属的物种间差异很大。该结果表明,RAPD标记可用于“栎”遗传多样性分析。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号