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首页> 外文期刊>African Journal of Biotechnology >In vitro antioxidant analysis of Achillea tenuifolia
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In vitro antioxidant analysis of Achillea tenuifolia

机译：中华A的体外抗氧化剂分析

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Achillea tenuifolia?(AT) is one of the most herbs are being used by people as a traditional medicinal remedy. Antioxidant activity of AT different extracts and total flavonoid and phenol levels in the extracts were investigated in this study. Plant extracts were prepared by maceration method using ethyl acetate, methanol and methanol-water (1:1). Folin Ciocalteu reagent in terms of gallic acid equivalent achieved the total phenol's content. AlCl3?was used as a reagent for flavonoid determination. Flavonoid content of the plant extracts obtained in terms of quercetin equivalent. DPPH radical scavenging effect of the extracts was determined by UV spectroscopy. Also in order to determine lipid peroxidation inhibition of the extracts of A. tenuifolia, ferric thiocyanate method with BHT, a synthetic reference standard, was carried out in this study. Phenol contents were 43.97 ± 0.034, 74.16 ± 0.55 and 106 ± 0.693 mg g-1?in the ethyl acetate, methanol and methanol-water extracts, respectively. Flavonoid amount obtained in the ethyl acetate, methanol and methanol-water extracts were 10.6 ± 1.85, 23.1 ± 0.5 and 190 ± 1.3 mg g-1, respectively. The percentage of DPPH radical scavenged by the most active extract (methanol-water) of A. tenuifolia was 92% at a concentration of 1 mgml-1?greater than 94% of BHT at 2 mgml-1. IC50?of methanol-water extract and BHT were 0.015 and 0.053 mgml-1, respectively. Lipid peroxidation inhibition was observed by the most polar extract of AT about 91.84%. Phenol and flavonoids content confirm the existence of more polar hydroxyl containing chemical structures in the plant. The potency of radical scavenging effect of methanol-water extract was about 3.5 times greater than synthetic antioxidant BHT. The inhibitory activity of the extracts on the lipid peroxidation of linoleic acid in ferric thiocyanate test was also significant (> 90%). In this study we concluded that there is a direct relation between phenol and flavonoid content of plant extracts and the antioxidant activity. So that the greater amount of phenolic compounds leads to more potent radical scavenging and lipid peroxidation inhibition activities as it was observed in A. tenuifolia polar extract in the present study.
机译：Achillea tenuifolia？（AT）是被人们用作传统药物的最多草药之一。本研究研究了AT提取物的抗氧化活性以及提取物中总黄酮和苯酚的含量。通过浸没法使用乙酸乙酯，甲醇和甲醇-水（1：1）制备植物提取物。以没食子酸当量计的Folin Ciocalteu试剂达到了总酚的含量。 AlCl 3用作测定类黄酮的试剂。以槲皮素当量表示的植物提取物中的类黄酮含量。提取物的DPPH自由基清除作用通过紫外光谱法测定。另外，为了确定对青蒿的提取物的脂质过氧化抑制作用，在这项研究中进行了使用BHT（一种合成的参考标准）的硫氰酸铁方法。乙酸乙酯，甲醇和甲醇-水提取物中的苯酚含量分别为43.97±0.034、74.16±0.55和106±0.693 mg g-1?。在乙酸乙酯，甲醇和甲醇-水提取物中获得的类黄酮含量分别为10.6±1.85、23.1±0.5和190±1.3 mg g-1。在浓度为1 mgml-1？时，活性最强的非洲曲霉提取物（甲醇-水）清除的DPPH自由基的百分比为92％，大于2mgml-1的BHT的94％。甲醇-水提取物和BHT的IC50分别为0.015和0.053 mgml-1。通过最极性的AT提取物观察到脂质过氧化抑制为约91.84％。苯酚和类黄酮的含量证实了植物中存在更多极性含羟基的化学结构。甲醇-水提取物的自由基清除作用的效力是合成抗氧化剂BHT的约3.5倍。在硫氰酸铁测试中，提取物对亚油酸脂质过氧化的抑制作用也很显着（> 90％）。在这项研究中，我们得出结论，植物提取物中的苯酚和类黄酮含量与抗氧化活性之间存在直接关系。因此，如本研究中在tenuifolia极地提取物中观察到的，更多的酚类化合物导致更有效的自由基清除和脂质过氧化抑制活性。

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