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首页> 外文期刊>African Journal of Biotechnology >Kappa-casein gene polymorphism in Holstein and Iranian native cattle by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP)
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Kappa-casein gene polymorphism in Holstein and Iranian native cattle by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP)

机译：聚合酶链反应限制片段长度多态性（PCR-RFLP）技术在荷斯坦和伊朗本地牛中的κ-酪蛋白基因多态性

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摘要

Caseins amount to nearly 80% of the protein output in cow milk. Caseins are biologically important proteins and they are also a raw material for the cheese making industry. The aim of this study was to identify?kappa-casein genotype?inHolstein?and Iranian native cattle. DNA was extracted from 457 blood samples of 247 Holstein and 210 native cattle for identification and genotyping of kappa-casein gene by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) assay using?HindIII and?TaqI restriction endonucleases. The PCR product of the specific primer K-F and K-R gives the 379 bp specific band. Digestion of 379 bp fragment by restriction endonuclease?HindIII generated two fragments of 156 and 223 bp. Result of the cut with this enzyme indicate there genotypes AA, AB and BB in the samples. Also, the amplified DNA (379 bp) from the samples remained undigested by?TaqI?restriction enzyme. These findings suggest that BB genotype could be a good factor for increase of fat and protein content of milk.
机译：酪蛋白占牛奶中蛋白质产量的近80％。酪蛋白是生物学上重要的蛋白质，它们也是干酪制造行业的原材料。这项研究的目的是鉴定荷斯坦和伊朗本地牛的“κ酪蛋白基因型”。用HindIII和TaqI限制性核酸内切酶通过聚合酶链反应限制性片段长度多态性（PCR-RFLP）法从247只荷斯坦牛和210只本地牛的457份血液样本中提取DNA，以鉴定和鉴定kappa-酪蛋白基因。特异性引物K-F和K-R的PCR产物给出了379bp的特异性条带。用限制性核酸内切酶HindIII消化379bp的片段，产生两个156bp和223bp的片段。用该酶切割的结果表明样品中存在AA，AB和BB基因型。另外，从样品中扩增出的DNA（379bp）仍未被“ TaqI”限制性酶消化。这些发现表明，BB基因型可能是增加牛奶中脂肪和蛋白质含量的好因素。

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