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首页> 外文期刊>African Journal of Biotechnology >Oxidant and solvent stable alkaline protease from Aspergillus flavus and its characterization
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Oxidant and solvent stable alkaline protease from Aspergillus flavus and its characterization

机译：黄曲霉的氧化剂和溶剂稳定碱性蛋白酶及其表征

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摘要

The increase in agricultural practices has necessitated the judicious use ofagricultural wastes into value added products. In this study, an extracellular, organic solvent and oxidant stable, serine protease was produced by?Aspergillus flavusMTCC 9952 under solid state fermentation. Maximum protease yield was obtained when the strain was grown under wheat bran and corn cob mixture (1:1) incubated for 48 h at pH 9.0 and temperature 37°C with 50% of initial moisture content. The partially purified enzyme showed wide range of pH optima (8.0-12.0) and pH stability (7.0-12.0), whereas, optimum temperature was 40°C and was stable over a wide range of temperature 30-45°C. The protease was extremely stable towards several organic solvents. The enzyme retained 80% of its original activity in the presence of non ionic and ionic surfactants and 100% with 10% H2O2?after 1 h of incubation at 30°C. In addition, the enzyme showed excellent compatibility with some commercial powder detergents. The compatibility of our protease with several detergents, oxidants and organic solvents suggests its possible use in detergent industry and peptide synthesis.
机译：随着农业实践的增加，必须明智地将农业废物用于增值产品。在这项研究中，由黄曲霉MTCC 9952在固态发酵下生产出一种细胞外的，有机溶剂和氧化剂稳定的丝氨酸蛋白酶。当菌株在麦麸和玉米芯混合物（1：1）下生长，在pH 9.0和温度37°C下以50％的初始水分温育48小时时，可获得最大的蛋白酶产量。部分纯化的酶显示出最适pH（8.0-12.0）和pH稳定性（7.0-12.0），而最适温度为40°C，并且在30-45°C的宽温度范围内稳定。蛋白酶对几种有机溶剂非常稳定。该酶在非离子和离子表面活性剂的存在下保留了其原始活性的80％，在30°C孵育1小时后保留了100％的10％H2O2 +。另外，该酶与某些市售粉末洗涤剂显示出极好的相容性。我们的蛋白酶与几种去污剂，氧化剂和有机溶剂的相容性表明它可能在去污剂工业和肽合成中使用。

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