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首页> 外文期刊>African Journal of Biotechnology >High efficacy of fibroblast and hepatocyte growth factors on the in vitro blastocyst production of post-thaw mouse two-cell embryo: The cryotop method
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High efficacy of fibroblast and hepatocyte growth factors on the in vitro blastocyst production of post-thaw mouse two-cell embryo: The cryotop method

机译：成纤维细胞和肝细胞生长因子对融化后小鼠两细胞胚胎体外胚泡产生的高效作用：冷冻切片法

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摘要

There is great need to improve our understanding of what increases an embryo’s development potential, after vitrification-thawing processes. For this subject, 358 two-cell stage embryos were collected from oviduct of pregnant two-day old mice and vitrified. After thawing, embryos were cultured in?Tyrode's?(T6) medium supplemented with different doses of fibroblast growth factor (FGF; 0, 10, 20, 50 and 100 ng/ml) and hepatocyte growth factor (HGF; 0, 10, 20, 50 and 100 ng/ml) until the blastocyst stage. To determine quality of blastocysts, blastocysts were stained with hoechst and propidium iodide. After culture for 24 h, 92.65% of treated embryos with 20 ng/ml of FGF had higher (P64 cells had a significantly higher inner cell mass (ICM) in comparison to the control group (P< 0.01). In conclusion, in this experiment, addition of growth factors in the culture had favorable effects on post-thawed cleavage of vitrified 2-cell embryos and blastocyst quality.
机译：在玻璃化解冻过程之后，非常需要增进我们对增加胚胎发育潜力的理解。对于该受试者，从怀孕两天大的小鼠的输卵管中收集了358个两细胞期胚胎并进行了玻璃化。解冻后，将胚胎在补充有不同剂量成纤维细胞生长因子（FGF; 0、10、20、50和100 ng / ml）和肝细胞生长因子（HGF; 0、10、20）的Tyrode'（T6）培养基中培养。 ，50和100 ng / ml）直至胚泡阶段。为了确定胚泡的质量，将胚泡用赫斯特和碘化丙啶染色。培养24小时后，有92.65％的20 ng / ml FGF处理过的胚胎具有更高的含量（P64细胞的内部细胞质量（ICM）与对照组相比明显更高（P <0.01）。实验中，在培养物中添加生长因子对解冻后的玻璃化2细胞胚胎裂解和胚泡质量具有良好的影响。

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