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Uptake, accumulation and some biochemical responses in Raphanus sativus L. to zinc stress

机译:萝卜(Raphanus sativus L.)对锌胁迫的吸收,积累和一些生化反应

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The responses of radish (Raphanus sativus?L.) to increasing concentrations of ZnCl2?(1, 5 and 10 mM) in Hoagland nutrient medium were studied. Under the conditions of these increasing zinc concentrations, the highest zinc accumulation was obtained in the roots of the plants treated with 10 mM applications. The zinc concentration in the vegetative parts, was highest in the root and was lowest in the cotyledons. The highest bioconcentration factor (BCF) value was detected in the roots in 5 mM zinc applications. When compared with the control, total zinc uptake was observed to increase in 1, 5 and 10 mM ZnCl2?treatments. The total accumulation rate (TAR) for zinc was highest in 10 mM ZnCl2?treatment, while the lowest TAR was observed in radish plants exposed to 1 mM ZnCl2. Plants treated with 5, 10 mM ZnCl2?showed significant decreases in chlorophyll (Chl a, Chl b, Chl a/b) and carotenoid content compared with the control. Peroxidase (POD) activity especially in radish roots increased significantly with increasing concentrations of ZnCl2?(5 and 10 mM)?while the total protein amount decreased when compared with the control. The results of this study showed that, radish plants could tolerate the negative effects of zinc stress up to 1 mM ZnCl2?concentration and that in zinc concentrations of 5 mM and above toxic effects were existent.
机译:研究了萝卜(Raphanus sativus?L。)对Hoagland营养培养基中ZnCl2?(1、5和10 mM)浓度增加的响应。在这些锌浓度增加的条件下,在用10 mM施用量处理过的植物根部获得了最高的锌累积量。营养部位的锌浓度在根中最高,在子叶中最低。在5 mM的锌施用中,根部检测到最高的生物浓缩因子(BCF)值。与对照相比,在1、5和10mM ZnCl 2处理中观察到总的锌吸收增加。锌的总累积速率(TAR)在10 mM ZnCl2?处理中最高,而在暴露于1 mM ZnCl2的萝卜植物中,TAR最低。与对照相比,用5、10 mM ZnCl2处理的植物的叶绿素(Chl a,Chl b,Chl a / b)和类胡萝卜素含量显着降低。与对照相比,随着ZnCl2α(5和10 mM)?浓度的增加,尤其是萝卜根中的过氧化物酶(POD)活性显着增加,而总蛋白质量则减少。这项研究的结果表明,萝卜植物可以忍受高达1 mM ZnCl2?浓度的锌胁迫的负面影响,并且在5 mM及以上的锌浓度下存在毒性作用。

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