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首页> 外文期刊>African Journal of Biotechnology >Cell number as an important variable in optimising inoculum age and size in yeast cultivation
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Cell number as an important variable in optimising inoculum age and size in yeast cultivation

机译：细胞数是优化酵母培养中接种物的年龄和大小的重要变量

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The growth lag phase during microbial fermentation reduced productivity and equipment efficiency. Inoculum age and size were the two important variables considered in fermentation optimisation. Previous studies investigated these two variables separately, in contrast to the orthogonal or use of response surface modelling method by recent papers. In nearly all cases, the inoculum from the late exponential or early stationary phase was selected as optimal. In the current study, the authors optimised inoculum conditions using a strategy that combined inoculum age and size as inoculum cell number to shorten the lag phase in yeast cultivation. Inoculum from the middle exponential phase (7th?h) exhibited priority in activity and adaptability. This condition was confirmed by inoculating the resuspended cell sediments into fresh potato dextrose medium. The results indicate that biomass (cell number for single cell microbes and wet/dry cell weight for filamentous strains), rather than inoculum age and size, should be considered during inoculum condition optimisation.
机译：微生物发酵过程中的生长滞后阶段降低了生产率和设备效率。接种物的年龄和大小是发酵优化中考虑的两个重要变量。以前的研究分别研究了这两个变量，这与最近的论文中正交或使用响应面建模方法相反。在几乎所有情况下，指数晚期或静止早期的接种物均被选为最佳。在当前的研究中，作者使用一种结合接种物年龄和大小作为接种物细胞数的策略来优化接种物条件，以缩短酵母培养的滞后阶段。中指数期（7th？h）的接种物表现出活性和适应性优先。通过将重悬的细胞沉淀物接种到新鲜的马铃薯葡萄糖培养基中可以确认这种情况。结果表明，在优化接种条件时，应考虑生物量（单细胞微生物的细胞数和丝状菌株的干/干细胞重量），而不是接种物的年龄和大小。

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