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首页> 外文期刊>African Journal of Biotechnology >Effect of polyethylene glycol and mannitol on somatic embryogenesis of pigeonpea, Cajanus cajan (L.) Millsp.
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Effect of polyethylene glycol and mannitol on somatic embryogenesis of pigeonpea, Cajanus cajan (L.) Millsp.

机译：聚乙二醇和甘露醇对木豆Cajanus cajan（L.）Millsp体细胞胚发生的影响。

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A protocol for high frequency somatic embryogenesis (SE) of pigeonpea,?Cajanus cajan?(L.) Millsp. was worked out. The age of the source seedlings for explants and type of explants were found to influence the callusing response. Concentration of phytohormones and growth factors were optimized for developing embryogenic callus consisting of globular cells with dense cytoplasm. Embryo axis or callus derived from such explants, when exposed to dehydration stress imposed by polyethylene glycol and osmotic stress created by D-mannitol were found to produce high frequency somatic embryos. Embryo axis from 3-day old sprouts or 28-day old callus derived from the embryo axis explants incubated for 4 h either in 4% polyethylene glycol (w/v) or 0.7 M mannitol were found optimal for SE. After exposure to stress incubation, explants or callus were cultured on semisolid?Murashige and Skoog (MS)?+?2,4-dichlorophenoxy acetic acid (2,4-D)?(5 μM) + glutamine (0.03 mM) and the cultures formed only globular and heart shaped somatic embryos. The early stage somatic embryos subsequently developed into torpedo and cotyledonary somatic embryos in MS liquid medium supplemented with 2,4-D (2 μM), abscisic acid (3 μM) and glutamine (0.03 mM). The somatic embryos were converted into plantlets in hormone-free half-strength MS semisolid medium and subsequently established in garden soil.
机译：木豆的高频体细胞胚发生（SE）的协议，“ Cajanus cajan”（L.）Millsp。被解决了。发现外植体的原始幼苗的年龄和外植体的类型会影响愈伤组织的应答。优化植物激素和生长因子的浓度，以开发由具有稠密细胞质的球状细胞组成的胚性愈伤组织。当暴露于聚乙二醇施加的脱水胁迫和D-甘露醇产生的渗透胁迫时，发现源自此类外植体的胚轴或愈伤组织可产生高频体细胞胚。发现在4％聚乙二醇（w / v）或0.7 M甘露醇中孵育4 h的3天大的芽苗或28天大的愈伤组织的胚轴最适合SE。暴露于胁迫下后，在半固体？Murashige和Skoog（MS）？+？2,4-二氯苯氧基乙酸（2,4-D）？（5μM）+谷氨酰胺（0.03 mM）上培养外植体或愈伤组织。培养仅形成球状和心形的体细胞胚。早期体细胞胚随后在补充有2,4-D（2μM），脱落酸（3μM）和谷氨酰胺（0.03 mM）的MS液体培养基中发育成鱼雷和子叶体细胞胚。体细胞胚在无激素半强度MS半固体培养基中转化为小植株，随后在花园土壤中建立。

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