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Significance of secondary metabolites and enzymes secreted by Trichoderma atroviride isolates for the biological control of Phomopsis canker disease

机译:木霉阿特罗韦德分离物分泌的次生代谢产物和酶对于防治枯萎病菌的生物学意义

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Phomopsis theae?Petch, the causative agent of Phomopsis canker diseases, is one of the major constrain in tea plants. The present study deals with development of efficient biological control agent against canker based on the secretion of several secondary metabolites and defense enzymes under?in vitro?conditions. Among the isolates evaluated, the antifungal compounds obtained from Tv1?Trichoderma atroviride?was able to inhibit the growth of pathogen and showed 100% inhibition at concentration of 500 ppm. Synthesis of extracellular enzymes such as amylase, cellulase, polygalacturonase, protease and?chitinase correlate with the antagonist activity of the isolates and maximum enzymatic activity was observed for Tv1 isolates followed by Tm3 and Tc3 isolates. The antifungal compounds responsible for the biological control activity were characterized through gas chromatography - mass spectroscopy (GC-MS) analysis.
机译:枯萎病的致病因子,Phophpsis theae?Petch,是茶树的主要限制之一。本研究基于体外条件下几种次生代谢产物和防御酶的分泌,研究了有效的防治溃疡病的生物防治剂。在评估的分离物中,得自Tv1?Atroviride Atroviride?的抗真菌化合物能够抑制病原体的生长,并在500 ppm浓度下表现出100%的抑制作用。细胞外酶的合成,例如淀粉酶,纤维素酶,聚半乳糖醛酸酶,蛋白酶和几丁质酶,与分离物的拮抗活性相关,并且在Tv1分离物,随后是Tm3和Tc3分离物中观察到最大的酶活性。通过气相色谱-质谱(GC-MS)分析对负责生物防治活性的抗真菌化合物进行了表征。

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