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Studies on amylase activity of an amylolytic bacterium isolated from estuarine soil

机译：河口土壤淀粉分解菌淀粉酶活性的研究

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Diverse microscopic, macroscopic and biochemical analysis of a starch degrading amylolytic bacterial strain isolated from the soil sample of Rajakkamangalam estuary, Kanyakumari district, Tamil Nadu, India, revealed its identity to the genus?Bacillus.Maximum growth was observed at 12 h when the bacteria was cultured in minimal agar media with 2% starch maintained for 12 h, at 37°C and pH 5.5, 7.5 and 8.5. Highest enzyme action as revealed by glucose production in the media was observed at pH 8.0, temperature 30°C and in the presence of 10 mM calcium (Ca). Enzyme activity gradually got reduced with the addition of increasing concentrations of ethylenediaminetetraacetic acid (EDTA), confirming the need for calcium for enzyme action. The amylase produced in the medium was isolated by centrifugation and partially purified by ammonium sulphate fractionation followed by dialysis.
机译：从印度泰米尔纳德邦Kanyakumari区Rajakkamangalam河口土壤样品中分离出的降解淀粉的淀粉分解菌菌株的各种微观，宏观和生化分析表明，它与芽孢杆菌属相同。当细菌在12 h达到最大生长在37℃和pH 5.5、7.5和8.5下，在含2％淀粉的基本琼脂培养基中培养12小时。在pH 8.0，温度30°C和存在10 mM钙（Ca）的情况下，观察到了培养基中葡萄糖生成所显示的最高酶作用。随着乙二胺四乙酸（EDTA）浓度的增加，酶的活性逐渐降低，这证实了钙对于酶作用的必要性。通过离心分离培养基中产生的淀粉酶，并通过硫酸铵分级分离，然后进行透析来部分纯化。

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