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首页> 外文期刊>African Journal of Biotechnology >Novel polymorphism of the bovine fat mass and obesity-associated (FTO) gene are related to backfat thickness and longissimus muscle area in five Chinese native cattle breeds
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Novel polymorphism of the bovine fat mass and obesity-associated (FTO) gene are related to backfat thickness and longissimus muscle area in five Chinese native cattle breeds

机译：五个中国本地牛品种的牛脂肪量和肥胖相关（FTO）基因的新型多态性与背脂厚度和背最长肌面积有关

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摘要

In this study, genetic variation of the fat mass and obesity-associated gene (FTO) was detected by PCR-SSCP and DNA sequencing in 618 individuals from five Chinese indigenous cattle breeds, and their genetic effects on meat quality traits were evaluated. The results showed that a novel single nucleotide polymorphism C1071T was detected in exon 5 and the allelic frequencies for the C and T alleles of the five breeds were 0.666/0.334, 0.583/0.417, 0.631/0.369, 0.653/0.347 and 0.689/0.311, respectively. Animals with CT genotype had higher mean values for backfat thickness than those with CC or TT genotypes (P < 0.01). Individuals with CC or CT genotypes had higher longissimus muscle area than those with TT genotype (P < 0.05). The FTO gene may be a candidate gene for identifying differences in meat quality traits and therefore, could be applied to marker-assisted selection of native Chinese cattle breeds.
机译：在这项研究中，通过PCR-SSCP和DNA测序检测了来自五个中国本土牛品种的618个个体的脂肪量和肥胖相关基因（FTO）的遗传变异，并评估了它们对肉质性状的遗传影响。结果表明，在第5外显子中检测到一个新的单核苷酸多态性C1071T，这5个品种的C和T等位基因的等位基因频率分别为0.666 / 0.334、0.583 / 0.417、0.631 / 0.369、0.653 / 0.347和0.689 / 0.311，分别。与CC和TT基因型的动物相比，CT基因型的动物的背脂厚度平均值更高（P <0.01）。 CC或CT基因型个体的长肌面积比TT基因型个体高（P <0.05）。 FTO基因可能是鉴定肉质性状差异的候选基因，因此可以用于标记辅助选择中国本地牛品种。

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