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Development of molecular marker of fatty acid-binding proteinFABP gene associated with longissimus muscle area and backfat thickness in Korean cattle
Development of molecular marker of fatty acid-binding proteinFABP gene associated with longissimus muscle area and backfat thickness in Korean cattle
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机译：脂肪酸结合蛋白FABP基因分子标记与长牛背肌面积和背脂厚度的关系
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摘要
The present invention is to identify the object-specific genetic capacity difference with respect to a very important baechoe muscle area and backfat thickness in the economic yield grade determines the item to determine the carcass and carcass amount of objects as the main six kinds of improved target traits of beef at an early stage fatty acid binding protein as a molecular marker using selection method, as the intracellular protein has a function to transport the fatty acids in the fatty acid oxidation from the cell membranes, phospholipids or triacylglycerols (triacylglycerol) synthesis place and fatty acid concentration cell particularly the fat metabolism control action ( fatty acid-binding protein, FABP) gene of restriction fragment polymorphism (restriction fragment length polymorphism; RFLP) analysis and beef in carcass traits of baechoe muscle area and backfat thickness associated with RFLP molecular markers developed by Cattle genetic capacity improvement and the amount of the conductor and the conductor and includes the information for utilization as a tool for predicting the starting high beef prematurely. ; In the present invention, to produce a primer, including beef heart fatty acid binding protein (H-FABP) intron (Intron) 1 single specific region of the gene amplified by PCR, a result of the cloning and analysis of the gene construct through the nucleotide sequence analysis, in this region one single nucleotide polymorphism; found a (single nucleotide polymorphism SNP) site and, of these single nucleotide polymorphisms were analyzed in association with the beef meat quality and carcass traits. ; That is, identify a single nucleotide polymorphism (SNP) site that occurred during the total base sequence of the 302bp size, including intron 1, one specific region of the bovine H-FABP gene from the 64th nucleotide sequence with cytosine (C) ↔ thymine (T) base substitution It was. This was cut to a single nucleotide polymorphism sequence site with a restriction enzyme TspRⅠ using the PCR-RFLP technique, and detects the three genotypes (C / C, C / T and T / T). And among the C / C genotype it is associated with a significantly higher backfat thickness of the beef, and T / T and C / T genotype was found a new fact that is associated with increased muscle area and significant baechoe. ; Accordingly, the present invention Hanwoo of backfat thickness (backfat thickness) and baechoe muscle area (Longgissimus muscle area) to the significant impact on heart fatty acid binding protein (H-FABP) gene in baechoe eye area is spacious and backfat thickness SNP genotyping is to use a thin amount of genetic improvement and the ability of the conductor, and the conductor, regardless of the age and sex of bovine develop DNA markers that can predict higher beef starter early on. ; Bovine heart fatty acid binding protein (H-FABP), sequencing, PCR-RFLP analysis, baechoe muscle area, fat thickness associated DNA markers elsewhere
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