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首页> 外文期刊>African Journal of Biotechnology >Molecular detection of TEM broad spectrum -lactamase in clinical isolates of Escherichia coli
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Molecular detection of TEM broad spectrum -lactamase in clinical isolates of Escherichia coli

机译：大肠杆菌临床分离株中TEM广谱-内酰胺酶的分子检测

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摘要

Resistance to β-lactam antibiotics, along with clinical isolates, frequently results to production of β-lactamase enzymes. In recent years, the production of extended spectrum β-lactamases (ESBLs) among clinical isolates, especially Escherichia coli has greatly increased. On the other hand, β lactamase genes have several subfamilies, and designing universal primers could be valuable to detect all of them. The beta lactamase enzyme producing E. coli, resistant to β-lactam antibiotics, created many problems for the patients. The TEM gene is responsible for β-lactamase resistance. The purpose of this study was to find out the percentage of E. coli strains that carry TEM in genes. In total, 500 clinical samples were collected from different Hospitals in Tehran. All the samples were isolated on EMB and MacConkey agar and incubated at 37°C for 24 h. The identification was carried out by conventional biochemical tests. Out of the 500 samples,?200 were identified as E. coli. The TEM gene was determined by PCR method on the isolates, which were already identified as Phenotypic by disk diffusion agar and combined disk. Out of the 200 isolated E. coli strains, 128 (64%) were producing ESBls. The PCR results show that 74 isolates of E. coli (57.8%) had the TEM gene. Our findings show that the majority of the ESBL positive clinical isolates of E. coli carried the TEM gene.
机译：对β-内酰胺抗生素的耐药性以及临床分离株经常导致产生β-内酰胺酶。近年来，临床分离株，尤其是大肠杆菌中，超广谱β-内酰胺酶（ESBLs）的产生大大增加。另一方面，β-内酰胺酶基因有几个亚科，设计通用引物可能对检测所有这些都有用。产生β-内酰胺酶的大肠杆菌对β-内酰胺类抗生素具有抗药性，给患者带来了许多问题。 TEM基因负责β-内酰胺酶的耐药性。这项研究的目的是找出在基因中携带TEM的大肠杆菌菌株的百分比。总共从德黑兰的不同医院收集了500个临床样本。在EMB和MacConkey琼脂上分离所有样品，并在37℃下孵育24小时。通过常规生化测试进行鉴定。在500个样本中，有200个被鉴定为大肠杆菌。通过PCR方法对分离物鉴定TEM基因，该分离物已被圆盘扩散琼脂和组合圆盘鉴定为表型。在200株分离的大肠杆菌菌株中，有128株（64％）正在生产ESB1。 PCR结果显示74株大肠杆菌（57.8％）具有TEM基因。我们的发现表明，大肠杆菌的大多数ESBL阳性临床分离株均带有TEM基因。

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