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Optimized system for plant regeneration of watermelon (Citrullus lanatus Thumb.)

机译:西瓜植物再生的优化系统(Citrullus lanatus Thumb。)

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The objective of this?study was to establish an efficient and reproducible?in vitroplant regeneration for?Citrullus lanatus?cv. Zaojia. To achieve optimal conditions for adventitious shoot induction, five explants (entire cotyledons, distal cotyledons, proximal cotyledons, cotyledonary node A and cotyledonary node B) were tested on MS medium supplemented with different concentrations and combinations of growth regulators (0 to 0.2 mg/L IAA and 1.0 to 5.0 mg/L BA), the results showed that entire cotyledons cultured in MS + BA (2.0mg/L) + IAA(0.2mg/L) achieved the highest regenerated rate (89.67%) and the optimal protocol screened?in this experiment had 7.69 ± 0.10 shoots per explants. Adventitious shoots were able to elongate both on MS medium with 0.2 mg/L KT and 0.2 mg/L NAA; IBA 0.3mg/L was found to be effective in the production of root.?Acclimatized plantlets?transferredto?pot resumed?growth,?and their stems and leaves elongated and expanded in one month.
机译:这项研究的目的是建立一种有效和可再现的体外培养Citrullus lanatus cv的植物。枣家为了获得诱导不定芽的最佳条件,在补充了不同浓度和生长调节剂组合(0至0.2 mg / L)的MS培养基上测试了五个外植体(整个子叶,远端子叶,近端子叶,子叶节A和子叶节B)。 IAA和1.0至5.0 mg / L BA),结果表明,在MS + BA(2.0mg / L)+ IAA(0.2mg / L)中培养的整个子叶达到了最高的再生率(89.67%),并且筛选了最佳方案在该实验中,每个外植体有7.69±0.10个芽。不定芽能够在含0.2 mg / L KT和0.2 mg / L NAA的MS培养基上延长;发现IBA 0.3mg / L对根的产生有效。将已驯化的小植株转移到盆中恢复生长,其茎和叶在一个月内伸长和扩展。

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