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首页> 外文期刊>African Journal of Biotechnology >Metabolic flux distribution and mathematical models for dynamic simulation of carbon metabolism in Escherichia coli
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Metabolic flux distribution and mathematical models for dynamic simulation of carbon metabolism in Escherichia coli

机译：大肠杆菌碳代谢动态模拟的代谢通量分布和数学模型

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A simple model?was build for the metabolic flux determination based on published articles. A method for metabolic flux determination by carbon labeling experiments was described and developed here in the first part of this study that allows mathematical description relating the measured quantities and the intracellular fluxes. The described method was used to investigate the central carbon metabolism of?Escherichia coli.?In the second part of?this?study,?computer simulation?was made to study the dynamics of the intracellular metabolite concentrations?in?E. coli?in particular for?the glycolysis and pentose-phosphate pathway based on?the?kinetic rate equations. The model successfully simulates the main features of the time course without alteration of the experimentally determined parameters.?After simulation starts, the intracellular concentrations of ATP, PEP, PYR, G6P, F6P, NAD and 3PG decreased while FDP, 6PG, S7P, E4P AMP, GAP, ADP, NADH and NADPH increased for wild?E. coli.?These simulation results were also partly verified by experimental results.
机译：基于已发表的文章，建立了一个简单的模型来确定代谢通量。在本研究的第一部分中，本文描述并开发了一种通过碳标记实验确定代谢通量的方法，该方法可以对测量量和细胞内通量进行数学描述。所描述的方法用于研究大肠杆菌的中心碳代谢。在本研究的第二部分，进行了计算机模拟，以研究大肠杆菌中细胞内代谢物浓度的动态变化。大肠杆菌尤其适用于基于运动速率方程的糖酵解和戊糖磷酸途径。该模型成功模拟了时间过程的主要特征，而无需改变实验确定的参数。？模拟开始后，ATP，PEP，PYR，G6P，F6P，NAD和3PG的细胞内浓度降低，而FDP，6PG，S7P，E4P野生？E的AMP，GAP，ADP，NADH和NADPH增加。这些模拟结果也得到了实验结果的部分验证。

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