Co-suppression of sterol-regulatory element binding protein mediates etiolation in Arabidopsis thaliana

Yuzhi Qin; Xu Li; Ming Guo; Xingyao Xiong Yong Song; He Changzheng; Xuanming Liu

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Co-suppression of sterol-regulatory element binding protein mediates etiolation in Arabidopsis thaliana

机译：固醇调节元件结合蛋白的共抑制介导拟南芥中的黄化

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Arabidopsis plants were transformed with a chimeric construct containing expression cassettes for GFP election marker and CaMV 35S promoter-driven?At5g35220?cDNA, via Agro bacterium-mediated method. Two transformants produced?pigmentation deficient phenotype. Analysis revealed the decrease of chlorophyll in all etiolated plants. RT-PCR showed that, total?At5g35220?mRNA levels were greatly inhibited in co-suppression lines.?PORA?and?PORB?mRNA expression were influenced also in the mutants. It is found that, the?At5g35220?gene is responsive to both inhibitor and some?hormone?with regard to MVP/MEP pathway in our study.

机译：通过农杆菌介导的方法，用含有GFP选择标记表达盒和CaMV 35S启动子驱动的ΔAt5g35220ΔcDNA的嵌合构建体转化拟南芥植物。两个转化子产生色素沉着缺陷表型。分析显示所有黄化植物中叶绿素的减少。 RT-PCR显示，共抑制株中总的At5g35220mRNA水平受到显着抑制。突变体中的PORA和PORBmRNA表达也受到影响。发现在我们的研究中，At5g35220基因对MVP / MEP途径对抑制剂和某些激素都有反应。

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《African Journal of Biotechnology》 |2011年第29期|共7页
作者
Yuzhi Qin; Xu Li; Ming Guo; Xingyao Xiong Yong Song; He Changzheng; Xuanming Liu;
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中图分类生物工程学（生物技术）;
关键词
Etiolationco-suppressionprotochlorophyllide oxidoreductase gene (POR)light regulation;

机译：Etiolationco-protoprotoprotochlorophyllide oxyreductase基因（POR）光调节;

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1. Arabidopsis thaliana ethylene-responsive element binding protein (AtEBP), an ethylene-inducible, GCC box DNA-binding protein interacts with an ocs element binding protein [J] . Michael Buttner, Karam B. Singh Proceedings of the National Academy of Sciences of the United States of America . 1997,第11期

机译：拟南芥乙烯反应元件结合蛋白（AtEBP），一种乙烯诱导的GCC盒DNA结合蛋白与ocs元件结合蛋白相互作用
2. Protein kinase Cbeta mediates hepatic induction of sterol-regulatory element binding protein-1c by insulin. [J] . Yamamoto T, Watanabe K, Inoue N, Journal of Lipid Research . 2010,第7期

机译：蛋白激酶Cbeta通过胰岛素介导肝脏诱导的固醇调节元件结合蛋白1c。
3. Protein kinase Cbeta mediates hepatic induction of sterol-regulatory element binding protein-1c by insulin [J] . Journal of Lipid Research . 2010,第7期

机译：蛋白激酶Cbeta通过胰岛素介导肝脏诱导的固醇调节元件结合蛋白1c
4. A novel light stress-enhanced one-helix chlorophyll a/b-binding protein from Arabidopsis thaliana [C] . U Andersson, I Adamska International Congress on Photosynthesis . 2001

机译：来自拟南芥的新型光应激增强的单螺旋叶绿素A / B结合蛋白
5. Functional Characterization of Selected Chloroplast RNA-Binding Proteins from Arabidopsis thaliana [D] . Kuciński, Jan P. 2020

机译：拟南芥中选择的叶绿体RNA结合蛋白的功能表征
6. Arabidopsis thaliana ethylene-responsive element binding protein (AtEBP) an ethylene-inducible GCC box DNA-binding protein interacts with an ocs element binding protein [O] . Michael Büttner, Karam B. Singh 1997

机译：拟南芥乙烯反应元件结合蛋白（AtEBP）一种乙烯诱导的GCC盒DNA结合蛋白与ocs结合蛋白相互作用
7. Protein kinase Cbeta mediates hepatic induction of sterol-regulatory element binding protein-1c by insulin [O] . Yamamoto, Takashi, Watanabe, Kazuhisa, Inoue, Noriyuki, 2010

机译：蛋白激酶Cbeta通过胰岛素介导肝脏诱导的固醇调节元件结合蛋白1c

Co-suppression of sterol-regulatory element binding protein mediates etiolation in Arabidopsis thaliana

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