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首页> 外文期刊>African Journal of Biotechnology >Defense responses in tomato fruit induced by oligandrin against Botrytis cinerea
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Defense responses in tomato fruit induced by oligandrin against Botrytis cinerea

机译：寡糖蛋白诱导的番茄果实对灰葡萄孢的防御反应

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Oligandrin is known to induce resistance against a number of plant diseases. However, its effects on postharvest diseases are still unclear. The effects of oligandrin on the control of postharvest diseases in tomato fruit and its underlying mechanisms were investigated in this study. The treatment of tomato fruit with oligandrin (10 μg/ml) significantly reduced the incidence and severity of gray mould (caused by?Botrytis cinerea). After 5, 7 and 9 days of artificial inoculation, the relative cure effect was 60.5, 52.1 and 48.5%, respectively. The results from bio-assay indicated that the treatment stimulated the activity of the defense related enzymes. Phenylalanine ammonia-lyase (PAL), polyphenol oxidase (PPO) and peroxidase (POD) activity in the oligandrin-treated fruit was about 39.2, 69.6 and 52.6% higher than that in control on the 3rd day, respectively. Furthermore, mRNA level of the genes encoding pathogenesis-related proteins (PRs), such as?PR-2a(extracellular β-1, 3 -glucanase) and?PR-3a?(extracellular chitinase) in tomato fruit was about 2.7-fold and 4.6-fold above that of the control at the peak stage. The expression of LeERF2 and PR6, which confer an ethylene-dependent signaling pathway, were also significantly increased 6.6-fold and 3.6-fold by such treatment. These results indicate that oligandrin has the potential to control gray mould and it may play an important role in the induction of resistance to?B. cinerea?and the activation of the ethylene-dependent signaling pathway.
机译：已知寡配体诱导对许多植物疾病的抗性。但是，其对收获后疾病的影响仍不清楚。本研究探讨了寡糖配体对番茄果实采后病害控制的作用及其潜在机理。用oligandrin（10μg/ ml）处理番茄果实可显着降低灰霉病的发生率和严重程度（由灰葡萄孢菌引起）。人工接种5、7和9天后，相对治愈率分别为60.5、52.1和48.5％。生物测定的结果表明该处理刺激了防御相关酶的活性。用低聚糖处理的果实中的苯丙氨酸氨解酶（PAL），多酚氧化酶（PPO）和过氧化物酶（POD）活性分别比第3天的对照高约39.2、69.6和52.6％。此外，番茄果实中与病程相关蛋白（PRs）相关的编码基因，如？PR-2a（细胞外β-1、3-葡聚糖酶）和？PR-3a？（细胞外几丁质酶）的基因的mRNA水平约为2.7倍。在高峰期比对照高4.6倍。通过这种处理，赋予乙烯依赖性信号传导途径的LeERF2和PR6的表达也显着增加了6.6倍和3.6倍。这些结果表明，寡聚糖具有控制灰霉病的潜力，并且可能在诱导抗？B方面起重要作用。灰质和乙烯依赖性信号传导途径的激活。

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